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한국식물학회 / v.43, no.3, 2000년, pp.171-178 
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( Expressed Sequence Tags of Young Floral Buds and Characterization of a Bud-Preferential Lectin-Like cDNA from Pharbitis nil ) |
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| 초 록 |
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| To identify the genes involved in flower development, we analyzed 207 expressed sequence tags (ESTs) from a young floral bud cDNA library of Pharbitis nil. Of these, 81 clones (42%) showed significant homology to known protein sequences in the NCBI database. Four of these had not been reported previously in the plant kingdom, indicating that 1.9% of the ESTs were newly identified in plants. functional categorization revealed that the genes involved in metabolic pathways, such as glycolysis and photosynthesis, were most abundant. Reverse-northern and northern analyses showed that one clone, PnFP161, was expressed preferentially in floral buds. DNA sequence analysis indicated that this clone contained 147 bp of 5'-UTR, 264 bp of 3'-UTR, and an open reading frame of 233 amino acids, thereby sharing 33% identity with a lectin from Calystegia sepium. The C-terminal region of PnFP161 had well-conserved residue with that of the lectins. Southern blot analysis demonstrated that PnFP161 exists as a multigene family, |
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| 키워드 |
| cDNA;EST;floral bud;lectin;Pharbitis nil; |
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Journal of Plant Biology / v.43, no.3, 2000년, pp.171-178
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200011921106681)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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