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한국식물학회 / v.44, no.4, 2001년, pp.185-192 
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( Regeneration of Grape (Vitis labruscana cv. Kyoho) by Shoot-Tip Culture ) |
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| We investigated the optimal levels of growth regulators, culture media, and pH on callus growth and organogenesis of in-vitro cultured ‘Kyoho’ gapes. Calli were induced by culturing leaf blades on an MS basal medium supplemented with 1 mg/L BA and 0.01 mg/L 2,4-D. In addition, calli originating from the exocarp and mesocarp of grape fruits developed on MS media supplemented with 0.1 mg/L IAA, NAA, or 2,4-D, or with 0.2 mg/L BA. In testing the potential for plant regeneration from shoot tips on various media, we found that the Nitsch medium, with 1 mg/L BA, was optimal for caulogenesis. The type of shoot development depended on the pH of the medium, with vigorous multiple-shoot development occurring at pH 6.0, and single shoots forming at pH 5.0. Finally we were able to obtain rooted seedlings from the regenerated shoots that had been cultured on 1/4-strength Nitsch medium supplemented with 0.03 mg/L NAA. |
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| 키워드 |
| grapes;Kyoho;regeneration;shoot-tip culture; |
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Journal of Plant Biology / v.44, no.4, 2001년, pp.185-192
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200111921138854)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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