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한국식물학회 / v.44, no.4, 2001년, pp.193-198 
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( The Effect of the Recombinant Human Interleukin-2 Gene in Potato (Solanum tuberosum cv. Superior) ) |
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| 초 록 |
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| To examine the effect of the T·cell growth factor (human interleukin-2), we constructed a binary vector, pSSK-1, carrying the recombinant human interleukin-2 (rhIL-2) gene, and transferred it into Agrobacterium tumefaciens. Using this construct, we then transformed potato explants (Solanum tuberosum w. Superior), achieving 100% regeneration of shoots on a modified MS medium. Of the putative transformed shoots,81% rooted and were selected on 200 mg/L kanamycin. Both Southern and northern analyses verified the transformation events. An ELISA test also indicated that the rhIL-2 protein was produced from rhIL-2-transformed potatoes. To determine whether this protein was biologically active in the potato tells, we performed a biological assay using the 11-2 dependent cell line, CTLL-2. The suspension containing extract from the transformants showed significant proliferation of the IL-2 dependent CILL-2 cells, whereas tells did not proliferate in the nontransformed potato. We then grew the verified rhIL-2 transgenic potatoes in soil, and compared their performance with that of nontransgenic potatoes as well as those that had been transformed with GUS. Growth rates, as calculated from plant heights, were up to 50% higher than for either the nontransgenic or the GUS-transformed potatoes. Similar patterns were found with Arabidopsis thaliana plants treated in the same manner, All of these results suggest that rhll-2 may function as a growth factor in potato. |
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| 키워드 |
| Arahidopsis thaliana;potato (Solanum tuberosum cv. Superior);recombinant human interleukin-2 (rhIL-2);T-cell growth factor; |
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Journal of Plant Biology / v.44, no.4, 2001년, pp.193-198
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200111921138864)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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