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한국식물학회 / v.44, no.4, 2001년, pp.231-235 
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( High-Frequency Regeneration and Transformation of Raphanus sativus ) |
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| 초 록 |
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| We have achieved high-frequency shoot regeneration in radish (Raphanus sativus). Cotyledon explants from four-day-old seedlings were suitable for the effective induction of shoots on Murashige and Skeets (MS) medium containing 3.0 mg/L kinetin. We also determined that it was essential to include 1-to 2-mm petiole segments with the cotyledons for efficient induction. When the regenerated shoots were transferred to an MS liquid medium containing 0.1 mg/L NAA, roots formed within four weeks, and normal plant development ensued. We established a transformation protocol using an Agrobacterium binary vector that carries the GUS reporter gene. Preculturing the explants for 1 d in an MS medium containing 3.0 mg/L kinetin also increased efficiency, Five days of cocultivation proved best for delivering T-DNA into radish. Transformation frequencies of up to 52% were obtained in shoot induction media that contained 3.0 mg/L kinetin. |
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| 키워드 |
| Agrobacterium;GUS;plant regeneration;radish (Raphanus sativus);transformation; |
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Journal of Plant Biology / v.44, no.4, 2001년, pp.231-235
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200111921138925)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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