|
|
|
한국식물학회 / v.45, no.1, 2002년, pp.56-61 
|
( Plant Regeneration and Morphology during in-Vitro Organogenesis from Heloniopsis orientalis (Thunb.) C. Tanaka ) |
| ;;; ;;;
|
|
|
 |
|
| |
| 초 록 |
|
|
| Heloniopsis prientalis (Liliaceae) is an important horticultural crop native to Korea. Under natural conditions, germination is poor and plant growth is delayed. Therefore, we have developed a vegetative propagation method to pro duce plants with vigorous growth characteristics via tissue culture. Leaf tissues were cultured on MS basal media supplemented with growth regulators 2,,4-D, TDZ, BA, or zeatin. The regenerated shoots were then initiated directly from leaf explants on an MS medium containing either 0.1 to 1.0 mg/L 2,4-D or 0.1 to 3.0 mg/L BA. Healthy plantlets with adventitious roots were formed on the medium supplemented with 0.1 mg/L BA. TDZ triggered callus initiation without caulogenesis or rhizogenesis, and callus formation was better on the half-strength MS medium than on the full-strength medium. After the plants were acclimatized for one month at 4$^{circ}C$, they were successfully transferred to soil. In addition, we used LM and SEM to investigate shoot morphogenesis at various stages of differentiation, |
| |
| 키워드 |
| Heloniopsis orientalis;regeneration; |
| |
|
|
|
|
Journal of Plant Biology / v.45, no.1, 2002년, pp.56-61
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200211921054998)
언어 : 영어 |
|
| 논문 제공 : KISTI 한국과학기술정보연구원 |
|
|
|
|
|
|
