|
|
|
한국식물학회 / v.45, no.3, 2002년, pp.170-176 
|
( Histological Study of Callus Formation and Root Regeneration from Mung Bean (Vigna radiata W.) ) |
| ;;; ;;;
|
|
|
 |
|
| |
| 초 록 |
|
|
| We have established a reproducible culture system for callus formation and root development from juvenile stem segments of mung bean (Vigna radiata). In particular, we have studied the influence of plant growth regulators. Induction of calli from young stem explants was very effective on MS inorganic salts supplemented with 0.5 mg/L 2,4-D and 1.0 mg/L kinetin. In regenerating adventitious roots from callus tissues, we found that a combination of 0.75 mg/L NAA, 1.5 mg/L kinetin, and MS salts resulted in 20% efficiency, Histological examination showed that callus tissues originated from out-growths of the cambium rings through do-novo meristematic activity, Those rings were localized outside the vascular cambium. Adventitious roots that developed from root primordia originated from the center of the Callus masses. These primordia produced tracheid-like cells, which then became meristemoid cells for the cambium. Newly formed adventitious roots had the typical tetrarche actinostele type. |
| |
| 키워드 |
| meristemoid;organogenesis;rooting;Vigna radiata; |
| |
|
|
|
|
Journal of Plant Biology / v.45, no.3, 2002년, pp.170-176
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200211921373923)
언어 : 영어 |
|
| 논문 제공 : KISTI 한국과학기술정보연구원 |
|
|
|
|
|
|
