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한국식물학회 / v.45, no.3, 2002년, pp.161-169 
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( Thymine-Starvation Up-Regulates a Region of the Arabidopsis thaliana Chromosome III Containing Both the UMP Synthase Gene and a Proximal Gene Encoding a Novel F-Box Protein ) |
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| 초 록 |
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| We have cloned the Arabidopsis thaliana uridine 5＇-monophosphate synthase (UMP synthase) locus and characterized transcript levels of this gene as well as the transcript encoding a novel F-box protein found upstream of UMP synthase, Skp1-interacting partner 5 (SKIP5). The 3＇end of the Skips gene is 405 bp from the 5＇end of the UMP synthase gene. To determine whether these proximate genes are coordinately or independently regulated, we have used an RT-PCR method to quantitate the transcript levels of each gene relative to 18S ribosomal RNA. Previous work has demonstrated an up-regulation of the UMP synthase gene in tobacco callus in the presence of compounds such as 5-fluoro-orotate and aminopterin that induce thymine starvation. Here, we present results showing that both the UMP synthase and SKIP5 genes are coordinately up-regulated in the presence of fluoroorotic arid. |
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| 키워드 |
| Arabidopis thaliana;F-Box protein;fluoroorotic acid;Nicotiana plumbaginifofla;pyrimidine metabolism;SKIP5;thymine;UMP Synthase; |
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Journal of Plant Biology / v.45, no.3, 2002년, pp.161-169
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200211921373914)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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