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한국식물학회 / v.45, no.3, 2002년, pp.177-181 
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( Callus Induction and Plant Regeneration from Broccoli (Brassica oleracea var. italica) for Transformation ) |
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| 초 록 |
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| We evaluated the efficiency of callus intuition and plantlet regeneration from hypocotyl explants of broccoli (Brassica oleracea var. italica). The cultivars were ‘Marathon’,‘Greenbelt’, and ‘Shogun’. Transformation success was not affected by the presence of tobacco feeder-cell layers on the culture media. The frequency of shoot regeneration was greater from 10-d-old hypocotyls than from 14-d-old hypocotyls. Both ‘Marathon’ and ‘Greenbelt’ had higher potentials for tissue regeneration than did ‘Shogun’. We found that for transformation selection, the optimum concentration was either 50 mg/L kanamycin or 100 mg/L geneticin. |
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| 키워드 |
| broccoli;callus induction;hypocotyls;plant regeneration;tobacco-feeder-cell layers;transformation; |
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Journal of Plant Biology / v.45, no.3, 2002년, pp.177-181
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200211921373934)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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