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한국식물학회 / v.20, no.2, 1977년, pp.63-69 
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당근 배양세포의 생육에 미치는 수종 Hormone의 영향
( The Effects of Various Hormones on Growth of Carrot Tissue Culture ) |
| 강영희; 연세대학교 이과대학;
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| 초 록 |
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| Experiments were conducted to investigate the effects of several hormones on the growth of suspension culture of carrot (Daucus carota L.) cells, where changes in pH and the amount of $NH_4-Nin$ the medium were observed with regard to growth. A treatment with 2,4-D at $10^{-5}M$ resulted in a highest rate of growth; the hormone at this concentration caused an increase in dry weight by about 40 to 50% over the control, measured at a stationary phase. It was thus indicated that 2,4-D at $10^{-5}M$ provided the optimal condition for the suspension culture. Changes in pH of the medium were found to be affected by hormonal treatments during the first 2-3 days following the inoculation, after which the pattern of pH changes in hormone enriched media paralleled that of the control. Subsequent changes of $NH_4-N and NO_3-N$ from the medium by the cells, and also by growth of the cells. The uptake of $NH_4-N$ by the cells did not appear to be influenced by hormonal treatments. At a stationary phase, a considerable amout of $NO_3-N$ played a more important role than $NH_4-N$ in the growth of the carrot cell suspension culture. |
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| 키워드 |
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Journal of Plant Biology / v.20, no.2, 1977년, pp.63-69
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO197711920112711)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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