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한국식물학회 / v.22, no.4, 1979년, pp.95-100 
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gibberellic Acid의 작용기작에 관한 연구 I. $GA_{3}$에 의한 단백질의 생합성 및 인산화반응의 조절
( Studies on the Mechanisms of Gibberellic Acid Action I. Regulation of Protein Biosynthesis and Phosphorylation by Gibberellic Acid $_{3}$ ) |
| 심웅섭; 고려대학교 이과대학 생물학과;
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| As a part of the studies on the regulatory mechanism of gene expression by $GA_{3}$ , the effects of $GA_{3}$ on the protein biosynthesis and phosphorylation in maize seedlings were investigated. 1. The optimum concentration of $GA_{3}$ for the stimulation of the protein biosynthesis was 0.3mM. 2. The protein biosynthesis was remarkably increase by $GA_{3}$ during the germination. The reason for the decrease in the protein biosynthesis by 48hrs. after germination seems to be a staggered gene expression, and/or increases in protease and RNase activities. 3. The ratio of the amount of the newly synthesized protein in germinating seeds treated with $GA_{3}$ to the amount of proteins secreted into the endosperm was similar to that ratio in control. According to this result, it seems that $GA_{3}$ stimulates only the expression of certain definite genes. 4. By the treatment with $GA_{3}$, the rates of biosynthesis and phosphorylation of proteins were increased up to about 1.5 times during germination and 6 times by 72hrs. after germination, respectively. The ratio of the total soluble proteins to the phosphorpoteins considerably increased in the early germination stage (24hrs.) but decreased after 24hrs. According to the above mentioned results, the stimulation of the phosphorylation of proteins of $GA_{3}$ seems to be attributed to the increases in the activities of protein kinases. |
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Journal of Plant Biology / v.22, no.4, 1979년, pp.95-100
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO197911920113238)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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