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한국식물학회 / v.23, no.2, 1980년, pp.49-54 
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완두와 보리의 엽내세포 원형질체 분이 및 융합
( The Isolation and Fusion of Pea and Barley Mesophyll Protoplasts ) |
| 이광웅; 서울대학교 자연과학대학 식물학과;
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| 초 록 |
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| The optimal conditions for the protoplast isolation from the leaves of pea (Pisum sativum L. cv. Sparkle) and barley (Hordeum vulgare L. cv. Baecdong) were determined in order to achieve a somatic hybridization between two species. It was revealed that the use of 0.5M sorbitol as an osmoticum was appropriate for pea. The yield of intact protoplasts was the highest (40%) when pea leaves were incubated in the enzyme solution for 4 hours. In case of barley, the optimal concentrations of cellulase, pectinase and mannitol as the enzyme solution were 2%, 1% and 0.35M, respectively. And the yield of barley protoplasts was the highest(87%) when leaves were incubated in this enzyme solution for 3.5 hours. A fusion of protoplasts from pea and barley was induced by PEG treatment enriched with calcium salts within 60 minutes. |
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| 키워드 |
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Journal of Plant Biology / v.23, no.2, 1980년, pp.49-54
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198011920113344)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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