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Çѱ¹½Ä¹°ÇÐȸ / v.24, no.1, 1981³â, pp.13-19
°í·ÁÀλï(Panax ginseng C.A. Meyer) ¹è¾ç¼¼Æ÷ÀÇ »ýÀ° ¹× Áú¼Ò Èí¼ö¿¡ ¹ÌÄ¡´Â Ammonium Citrate¿Í Ammonium SuccinateÀÇ ¿µÇâ
( The Effects of Ammonium Citrate and Ammonium Succinate on the Growth of Cells and Nitrogen Absorption in Korean Ginseng Suspension Cultures )
±èÈ«¼º;±è¸í¿ø;¼Ò»ó¼·;°­¿µÈñ; ¿¬¼¼´ëÇб³ À̰ú´ëÇÐ »ý¹°Çаú;¿¬¼¼´ëÇб³ À̰ú´ëÇÐ »ý¹°Çаú;¿¬¼¼´ëÇб³ À̰ú´ëÇÐ »ý¹°Çаú;¿¬¼¼´ëÇб³ À̰ú´ëÇÐ »ý¹°Çаú;
 
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Ammonium citrate¿Í ammonium succinate°¡ °í·ÁÀλï(Panax ginseng C. A. Meyer) ¹è¾ç¼¼Æ÷ÀÇ »ýÀ° ¹× Áú¼Ò¼ººÐÀÇ Èí¼ö¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» Á¶»çÇϱâ À§ÇÏ¿© Murashige and Skoog ¹èÁö¿¡¼­ ammonium nitrate ´ë½Å¿¡ ³óµµ¸¦ ´Þ¸®ÇÏ¿© ammonium citrate¿Í ammonium succinate¸¦ ÷°¡ÇÑ MS¹èÁö¿¡ Àλï callus¸¦ ¾×ü ÁøÅÁ¹è¾çÇÏ¿© ´ÙÀ½°ú °°Àº °á·ÐÀ» ¾ò¾ú´Ù. Ammonium citrate 10mM°ú ammonium succinate 10mMÀ» ÷°¡ÇÑ ¹è¾ç±â¿¡¼­ »ýÀ°·®, NO$_3$-N Èí¼ö·® ¹× ÀüÁú¼Ò·®ÀÌ controlº¸´Ù ¸¹¾Ò°í, NH$_4$-N Èí¼ö·®Àº control°ú °°¾Ò´Ù. ¶ÇÇÑ °¢°¢ 5mM¾¿ ÷°¡ÇÑ °æ¿ì¿¡´Â °ÅÀÇ ¸ðµÎ control°ú º° Â÷À̰¡ ¾ø¾ú´Ù. °á·ÐÀûÀ¸·Î ammonium citrate 10mM°ú ammonium succinate 10mM÷°¡ÇÑ ¹è¾ç±â¿¡¼­´Â NO$_3$-N µîÀÇ Èí¼ö¸¦ ÃËÁøÇÏ¿© ¹è¾ç¼¼Æ÷ÀÇ ´ë»ç¸¦ ÃËÁø½ÃÄÑ »ýÀ°·®À» Áõ°¡½ÃŰ´Â °ÍÀ¸·Î »ý°¢µÈ´Ù. ±×¸®ÇÏ¿© ammonium citrate ¶Ç´Â ammonium succinateÀÇ ³óµµ¸¦ Á¶Àý °ø±ÞÇϸé ÀÎ»ï ¹è¾ç¼¼Æ÷ÀÇ Áõ½ÄÀ» ÃËÁø½Ãų ¼ö ÀÖ´Ù°í »ç·áµÈ´Ù.
In order to investigate the effects of ammonium citrate and ammonium succinate on the growth and absorption of nitrogen compounds supplied in the medium, Korean ginseng (Panax ginseng C. A. Meyer) calli were suspension cultured in MS medium with various concentrations of ammonium citrate and ammonium succinate. When Korean ginseng calli were cultured with 10 mM ammonium citrate, 10 mM ammonium succinate, and 10 mM ammonium nitrate (control) in MS media as the nitrogen sources, the growth, $NO_3$-N absorption and total nitrogen content of the Korean ginseng cells were greatest in the ammonium citrate and ammonium succinate concentrations. When Korean ginseng calli were cultured with 5 mM ammonium citrate and 5 mM ammonium succinate, the growth and nitrogen content were superior to those of the control: however, $NO_3$-N and $NH_4$-N absorptions were similar to those of the control. In conclusion, the 10 mM ammonium citrate and 10 mM ammonium succinate may be better able to facilitate the growth and $NO_3$
 
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Journal of Plant Biology / v.24, no.1, 1981³â, pp.13-19
Çѱ¹½Ä¹°ÇÐȸ
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198111922411077)
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