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한국식물학회 / v.29, no.3, 1986년, pp.197-205 
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Murashige와 Skoog 수정배지를 사용한 담배(Nicotiana tabacum L.) 재배종의 원형질체 배양
( Protoplast Culture in Five Cultivars of N. tabacum L. by Modified Murashige and Skoog Medium ) |
| 김상구; 서울대학교 자연과학대학 식물학과;
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| 초 록 |
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| Leaf mesophyll protoplasts from five cultivars of tobacco (N. tabacum L.) were cultured. The protoplasts did not survive in culture medium containing Murashige and Skoog inorganic salts for over 6 days. NH4NO3 and FeSO4.Na2EDTA concentration of Murashige and Skoog medium were toxic in tobacco leaf mesophyll protoplast culture. Therefore we investigated optimum condition in Murashige and Skoog medium. High plating efficiency was obtained by reducing the concentrations of NH4NO3 and FeSO4.Na2EDTA to 1/3 and 1/10, respectively, on the supplemented with 5$mu$M IAA, 0.5 $mu$M 2.4-D 5 $mu$M BAP. Plants were regenerated from protoplast-derived calluses. |
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| 키워드 |
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Journal of Plant Biology / v.29, no.3, 1986년, pp.197-205
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO198611920114049)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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