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한국식물학회 / v.52, no.5, 2009년, pp.453-461 
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( Overexpression of AtNHX1, a Vacuolar $Na^+/H^+$ Antiporter from Arabidopsis thalina, in Petunia hybrida Enhances Salt and Drought Tolerance ) |
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| 초 록 |
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| Plant $Na^+/H^+$ antiporter plays a critical role in plant salt tolerance. In this study, AtNHX1, a vacuolar $Na^+/H^+$ antiporter from Arabidopsis thalina, was introduced into Petunia hybrida using Agrobacterium-mediated methods. Polymerase chain reaction (PCR) and DNA gel blot analysis confirmed that AtNHX1 was successfully integrated into the P. hybrida genomes. Reverse transcription (RT)-PCR analysis indicated that AtNHX1 expressed highly in transgenic plants. It was found that the 35S::AtNHX1 transgenic P. hybrida plants showed much more resistant to high concentrations of NaCl and to water deprivation than the isogenic wild-type (WT) Petunia plants. The transgenic plants accumulated more $Na^+$, $K^+$, and proline in their leaf tissue than that of the WT Petunia plants and maintained high water contents and high ratio of $K^+/Na^+$. These results demonstrated that over-expression of the vacuolar $Na^+/H^+$ antiporter conferred both salt tolerance and drought tolerance to the transgenic Petunia plants. |
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| 키워드 |
| Drought tolerance;Petunia hybrida;Salt tolerance;Transgenic plants;Vacuolar $Na^+/H^+$ antiporter; |
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Journal of Plant Biology / v.52, no.5, 2009년, pp.453-461
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200912763361257)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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