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한국식물학회 / v.52, no.6, 2009년, pp.630-634 
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( Evaluation of Viability, Shedding Pattern, and Longevity of Pollen from Genetically Modified (GM) Herbicide-tolerant and Wild-type Zoysiagrass (Zoysia japonica Steud.) ) |
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| 초 록 |
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| Knowledge about pollen viability is important when evaluating the risk of genetically modified (GM) plants. Here, staining via iodine potassium iodide (IKI) or triphenyl tetrazolium chloride (TTC) could not distinguish between live and dead pollen from Zoysia japonica. Therefore, to obtain a reliable assessment of such viability and longevity, we developed an optimum germination medium containing 20% sucrose and 50 ppm $H_3BO_3$. Pollen grains transferred to the germination medium at about 1000 hours had a germination rate of >90%. Pollen was most predominantly shed at approximately 1000 hours, with viability declining to nearly 0% at 1200 hours. All germinability was lost within 150 min when stored at $25^{circ}C$. No significant difference was found between GM and non-GM plants in their pollen viability or longevity. |
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| 키워드 |
| Gene flow;Genetically modified (GM);Pollen;Zoysia japonica; |
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Journal of Plant Biology / v.52, no.6, 2009년, pp.630-634
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200915939076918)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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