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한국식물학회 / v.52, no.6, 2009년, pp.518-523 
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( Comparison of Endothelium-Dependent Vasorelaxation of Crude Ginsenosides from Mountain-Grown Ginseng and Red Ginseng ) |
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| 초 록 |
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| Mountain-grown ginseng (Panax ginseng C. A. Meyer; Sansam in Korean) is believed to possess more potent biological activity than red ginseng. This study examined the endothelium-dependent vasorelaxant effects and possible mechanisms of crude ginsenosides from adventitious roots of Korean mountain-grown ginseng (GS-ARMG) and red ginseng (GS-RG) in isolated rat aorta precontracted with norepinephrine. GS-ARMG (0.03~3.0 mg/mL) produced transient acute relaxation in a concentration-dependent manner, with a maximum relaxation (mean$pm$ SEM) of $90{pm}9%$ and a median effective concentration ($EC_{50}$) of $0.09{pm}0.07;mg/mL$. GS-ARMG displayed about 25-fold more potent activity than GS-RG (maximum relaxation $50{pm}4%$, $EC_{50}$ $2.34{pm}1.30;mg/mL$). Relaxations induced by both GS-ARMG (1.0 mg/mL) and GS-RG (1.0 mg/mL) were nearly abolished by endothelial ablation or pre-treatment with $N^G$-nitro-L-arginine, a nitric oxide synthase inhibitor, or by methylene blue, a soluble guanylate cyclase inhibitor. These inhibitory effects, however, revealed different sensitivity of GS-ARMG and GS-RG; the maximum relaxations attained were 30.38% and 13.17% that of untreated preparations, respectively, but indomethacin and cyclooxygenase inhibitors did not affect the response. None of the receptor antagonists, atropine, diphenhydramine, [D-$Pro^2$, D-$Trp^{7,;9}$]-substance P, or propranolol, caused any significant inhibition to GS-ARMG-induced relaxation; however, atropine or propranolol caused a 10% reduction in the relaxation, suggesting possible involvement of a muscarinic receptor or a $eta$-adrenoceptor in the GS-ARMG-induced relaxation. These results demonstrate that GS-ARMG produces endothelium-dependent relaxation of isolated rat aorta similar to that of GS-RG; increased nitric oxide production and increased vascular levels of cGMP in endothelial cells could contribute to the relaxation. However, GS-ARMG has more potent activity than GS-RG to relax isolated rat aorta though an active substance(s), which might be higher in mountain-grown ginseng due to the growing conditions on mountains or the processing during manufacture of GS-ARMG. These factors may contribute to understanding the biological beneficial effects of mountain-grown ginseng. |
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| 키워드 |
| Vasorelaxation;Adventitious root of mountain-grown ginseng;Endothelium-dependent;Crude Ginsenosides; |
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Journal of Plant Biology / v.52, no.6, 2009년, pp.518-523
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200915939076894)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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