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한국식물학회 / v.51, no.6, 2008년, pp.401-407 
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( Development of Selection Marker-free Transgenic Potato Plants with Enhanced Tolerance to Oxidative Stress ) |
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| 초 록 |
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| A binary vector devoid of a plant selection-marker gene (designated as pSSA-F) was constructed to overcome bio-safety concerns about genetically modified plants. This vector carried chloroplast-targeted superoxide dismutase (SOD) and ascorbate peroxidase (APX) genes under the control of an oxidative stress-inducible (SWPA2) promoter, and was utilized to transform potato (Solanum tuberosum L.). Integration of these foreign genes into transgenic plants was primarily performed via PCR with genomic DNA. Twelve marker-free transgenic lines were obtained by inoculating stem explants. The maximum transformation efficiency was 6.25% and averaged 2.2%. Successful integration of the SOD and APX genes rendered transgenic plants tolerant to methyl viologen-mediated oxidative stress at the leaf-disc and whole-plant levels. Our findings suggest that this technique for developing selection marker-free transgenic plants is feasible and can be employed with other crop species. |
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| 키워드 |
| genetic transformation;marker-free transgenic plants;oxidative stress;potato; |
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Journal of Plant Biology / v.51, no.6, 2008년, pp.401-407
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200812651529942)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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