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한국식물학회 / v.50, no.1, 2007년, pp.1-7 
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( Chloroplast-Targeted BrMT1 (Brassica rapa Type-1 Metallothionein) Enhances Resistance to Cadmium and ROS in Transgenic Arabidopsis Plants ) |
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| 초 록 |
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| Metallothioneins (MTs) are low-molecular-weight, cysteine-rich proteins that bind to heavy metals. Type-1 MTs function under various abiotic stresses, including exposure to the cadmium ion. We have now isolated the Brassica rapa type-1 metallothionein gene (BrMT1) using yeast systems, and have found that it confers resistance to Cd in otherwise Cd-sensitive yeast. Using a constitutive CaMV355 promoter and an RbsS transit peptide, we successfully targeted BrMT1 to the chloroplasts of Arabidopsis. Overexpression in either the chloroplasts or the cytosol effectively detoxified cadmium and $H_{2}O_{2}$ stresses in transgenic Arabidopsis. In particular, the chloroplast-targeted BrMT1 was associated with a significant reduction in paraquat-induced chlorosis and the accumulation of $H_{2}O_{2}$. This is the first report regarding the effects of type-1 MT1 targeted to chloroplasts. Our results suggest that this may be applicable to the development of plants with enhanced tolerance against environmental stresses. |
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| 키워드 |
| BrMT1;cadmium;DAB staining;$H_{2}O_{2}$;metallothioneins;ROS; |
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Journal of Plant Biology / v.50, no.1, 2007년, pp.1-7
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200712242660636)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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