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Çѱ¹½Ä¹°ÇÐȸ / v.50, no.1, 2007³â, pp.12-17

( Transcript Profile of Transgenic Arabidopsis Constitutively Producing Methyl Jasmonate )
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Using an Affymetrix $GeneChip^{(R)}$ containing 8300 oligonucleotide probes, we measured transcript levels in transgenic Arabidopsis overexpressing the jasmonate carboxyl methyltransferase gene (AtJMT). When compared with wild-type plants, 5-week-old transgenics exhibited significant alterations (more than a two-fold increase or decrease) in the expression levels of 168 genes. Among them, 80 were up-regulated, including those involved in defense, oxidative stress-tolerance, and senescence. In contrast, the expression of 88 genes, including those that function in photosynthesis and cold/drought-stress responses, was significantly down-regulated. Thus, endogenous generation of methyl jasmonate through AtJMT-overexpression modified the transcript levels of genes previously identified as being jasmonate-responsive. This result confirms that MeJA formation is a key control point for jasmonate-responsive gene expression in plants.
 
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gene transcript profile;jasmonic acid methyltransferase;methyl jasmonate;microarray;transgenic Arabidopsis;
 
Journal of Plant Biology / v.50, no.1, 2007³â, pp.12-17
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ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200712242660769)
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