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한국식물학회 / v.50, no.1, 2007년, pp.29-37 
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( Aptamers Improve the Expression of a Human Granulocyte-Macrophage Colony Stimulating Factor in Transgenic Arabidopsis thaliana Seeds ) |
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| 초 록 |
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| Domain IV nucleotides of human 7SL RNA comprise an approximately 50-nucleotide region with a highly conserved secondary structure consisting of two internal loops. We inserted these into a modified 3'-untranslated region of the human granulocyte-macrophage colony stimulating factor (hGM-CSF) gene. The expression vector, under the control of a tissue-specific promoter derived from the soybean ${alpha}'$ subunit of ${eta}-conglycinin$, was introduced into Arabidopsis thaliana by Agrobacteriummediated transformation. Expression of the recombinant hGM-CSF significantly improved, accounting for as much as 0.049% of the total soluble protein in the Arabidopsis siliques. Western blots showed that the molecular weight of this recombinant hGM-CSF was approximately 21 kD. In addition, TF-1 cell proliferation data demonstrated that the recombinant hGM-CSF was biologically active. Our results provide evidence that aptamers can strongly enhance gene expression. |
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| 키워드 |
| aptamers;human GM-CSF;TF-1 cell; |
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Journal of Plant Biology / v.50, no.1, 2007년, pp.29-37
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200712242660973)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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