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한국식물학회 / v.51, no.3, 2008년, pp.227-232 
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( Preferential Spreading of RNA Silencing Into the 3' Downstream Region of the Transgene in Tobacco ) |
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| 초 록 |
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| The amplification mechanism of short interfering RNAs (siRNAs) along the transgene sequence exists in RNA interference (RNAi). The RNA-dependent RNA polymerase synthesizes complementary RNAs by using the transgene mRNA as a template, and the secondary siRNAs are generated from the outside of primary RNAi target. Four independent RNAi vectors which produced primary siRNAs against distinct regions of the tobacco endoplasmic reticulum ${omega}-3$ fatty acid desaturase gene (NtFAD3) were transiently expressed in leaves of the NtFAD3-overexpressed transgenic plants. Regardless of the RNAi vector used, the secondary NtFAD3 siRNAs were generated preferentially from the 3' downstream region of the transgene. Secondary siRNAs from the 5' upstream region adjacent to the annealing site of primary siRNAs accumulated under the detection level. Our results suggest that different regulatory mechanisms are involved in the spreading of RNA silencing into 5' upstream and 3' downstream regions of the target sequence, respectively. |
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| 키워드 |
| RdRP;secondary siRNA;transitive RNAi${omega}-3$ fatty acid desaturase; |
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Journal of Plant Biology / v.51, no.3, 2008년, pp.227-232
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200822049838973)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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