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한국식물학회 / v.51, no.3, 2008년, pp.209-212 
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( Aeration Volume and Photosynthetic Photon Flux Affect Cell Growth and Secondary Metabolite Contents in Bioreactor Cultures of Morinda citrifolia ) |
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| 초 록 |
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| To improve their growth and secondary metabolite production, we cultured Morinda citrifolia leaf cells for 3 weeks in bioreactors with different aeration volumes (0.05, 0.1, 0.2, or 0.3 vvm; or 0.05/0.1/0.2/0.3 vvm, as increased at 5-d interval), and photosynthetic photon fluxes (PPF; 0, 15, 30, or $45{mu}moL;m^{-2}s^{-1}$). Cell growth was greatest ($15.6g;L^{-1}$ dry weight) at 0.3 vvm whereas the accumulation of secondary metabolites (total anthraquinones, phenolics, and flavonoids) was maximized at 0.1 vvm. A PPF of $15{mu}moL;m^{-2}s^{-1}$ accelerated the accumulation of both cell biomass and metabolites. Dark-culturing suppressed cell growth, while a high PPF ($45{mu}moL;m^{-2}s^{-1}$) inhibited metabolite biosynthesis. Further studies are required to understand the reason for differences in the effect of light on cell growth and secondary metabolite contents in M. citrifolia cell cultures. |
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| 키워드 |
| aeration volume;anthraquinones;cell culture;flavonoids;Morinda citrifolia;phenolics;photosynthetic photon flux (PPF); |
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Journal of Plant Biology / v.51, no.3, 2008년, pp.209-212
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200822049838969)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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