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한국식물학회 / v.51, no.3, 2008년, pp.202-208 
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( Molecular Characterization of New S-RNases ('$S_{31}$' and '$S_{32}$') in Apple (Malus ${ imes}$ domestica Borkh.) ) |
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| 초 록 |
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| Apple exhibits gametophytic self-incompatibility (GSI) that is controlled by the multiallelic S-locus. This S-locus encodes polymorphic S ribonuclease (S-RNase) for the pistil-part S determinant. Information about S-genotypes is important when selecting pollen donors for fruit production and breeding of new cultivars. We determined the S-genotypes of 'Charden' ($S_2S_3S_4$), 'Winesap' ($S_1S_{28}$), 'York Imperial' ($S_2S_{31}$), 'Stark Earliblaze' ($S_1S_{28}$), and 'Burgundy' ($S_{20}S_{32}$), by S-RNase sequencing and S-allele-specific PCR analysis. Two new S-RNases, $S_{31}$ and $S_{32}$, were also identified from 'York Imperial' and 'Burgundy', respectively. These new S-alleles contained the conserved eight cysteine residues and two histidine residues essential for RNase activity. Whereas $S_{31}$ showed high similarity to $S_{20}$ (94%), $S_{32}$ exhibited 58% (to $S_{24}$) to 76% (to $S_{25}$) similarity in the exon regions. We designed new S-allele-specific primers for amplifying $S_{31}$- and $S_{32}$-RNase-specific fragments; these can serve as specific gene markers. We also rearranged the apple S-allele numbers containing those new S-RNases. They should be useful, along with an S-RNase-based PCR system, in determining S-genotypes and analyzing new alleles from apple cultivars. |
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| 키워드 |
| apple;gametophytic self-incompatibility;Malus domestica;S-genotype;S-RNase; |
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Journal of Plant Biology / v.51, no.3, 2008년, pp.202-208
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200822049838967)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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