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한국식물학회 / v.51, no.3, 2008년, pp.186-191 
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( Production of Somatic Hybrids between Satsuma Mandarin (Citrus unshiu) and Navel Orange (Citrus sinensis) by Protoplast Fusion ) |
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| 초 록 |
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| We have regenerated allotetraploid plants that are interspecific somatic hybrids between Citrus sinensis Osbeck cv. Yoshida navel orange and Citrus unshiu Marc cv. Okitsu satsuma mandarin. Protoplasts isolated from 'Yoshida' leaves were chemically fused with callus-derived protoplasts from 'Okitsu'. After 6 months of culture, 102 plants were obtained. These hybrids were identified by differential leaf morphology, DNA fluorescence intensity, and DNA analysis. Ploidy analysis via the flow cytometry revealed that 15 of the 102 plants were tetraploids, with the rest being diploids that morphologically resembled their mesophyll parent. SRAP analysis confirmed that 9 of the tetraploid plants were allotetraploid somatic hybrids. These will be utilized as a possible pollen parents for improving seedy citrus cultivars, e.g., ponkan, mandarin, lemon and kumquat, in order to produce triploid seedless hybrids. |
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| 키워드 |
| allotetraploid;protoplast;satsuma mandarin;somatic hybrid;SRAP; |
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Journal of Plant Biology / v.51, no.3, 2008년, pp.186-191
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200822049838964)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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