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한국식물학회 / v.51, no.3, 2008년, pp.174-179 
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( Functional Complementation of a Perilla ${omega}-3$ Fatty Acid Desaturase under the Seed-specific SeFAD2 Promoter ) |
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| 초 록 |
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| Functional characterization of the fatty acid desaturase genes and seed-specific promoters is prerequisite for altering the unsaturated fatty acid content of oilseeds by genetic manipulation. The ${omega}-6$ fatty acid desaturase (FAD2) and ${omega}-3$ fatty acid desaturase (FAD3) catalyze extra-plastidial desaturation of oleic acid to linoleic acid and linoleic acid to linolenic acid, respectively. These are major constituents in seed storage oils. Here, we report the complementation of a perilla linoleic acid desaturase (PrFAD3) cDNA under the seed-specific sesame FAD2 (SeFAD2) promoter in the Arabidopsis fad3 mutant. PrFAD3 is functionally active and the SeFAD2 promoter is applicable for modifying fatty acid composition in developing seeds. Transient expression of the GUS gene under that promoter in the developing seeds and leaves of sesame, soybean, and corn via microprojectile bombardment indicated that the SeFAD2 promoter likely will be useful for altering the seed phenotypes of dicot and monocot crops. |
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| 키워드 |
| FAD2;FAD3${omega}-3$ fatty acid desaturase${omega}-6$ fatty acid desaturase;Perilla frutescens;seed-specific promoter; |
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Journal of Plant Biology / v.51, no.3, 2008년, pp.174-179
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200822049838960)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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