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한국식물학회 / v.53, no.2, 2010년, pp.134-141 
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( Cloning and Characterization of Functional Trehalose-6-Phosphate Synthase Gene in Maize ) |
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| 초 록 |
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| Trehalose is a non-reducing disaccharide of glucose that functions as a compatible solute in the stabilization of biological structures under heat and desiccation stress in bacteria, fungi, and some "resurrection plants". In the plant kingdom, trehalose is biosynthesized by trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). Over-expression of exogenous and endogenous genes encoding TPS and TPP is reported to be effective for improving abiotic stress tolerance in tobacco, potato, tomato, rice, and Arabidopsis. On the basis of bioinformatics prediction, we cloned a fragment containing an open reading frame of 2,820 bp from maize, which encodes a protein of 939 amino acids. Phylogenetic analysis showed that this gene belongs to the class I subfamily of the TPS gene family. Analysis of conserved domains revealed the presence of a TPS domain and a TPP domain. Yeast complementation with TPS and TPP mutants demonstrated that this protein has the activity of trehalose-6-phosphate synthase. Semi-quantitative RT-PCR and realtime quantitative PCR indicated that the expression of this gene is upregulated in response to both salt and cold stress. |
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| 키워드 |
| Maize;Trehalose-6-phosphate synthase;Salt stress;Cold stress; |
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Journal of Plant Biology / v.53, no.2, 2010년, pp.134-141
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO201020733090186)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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