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한국식물학회 / v.53, no.4, 2010년, pp.251-258 
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( Isolation and Characterization of a Putative Vacuolar $Na^+/H^+$ Antiporter Gene from Zoysia japonica L. ) |
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| 초 록 |
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| A $Na^+/H^+$ antiporter gene from Zoysia japonica (ZjNHX1) which is a member of plant NHX-genes family was cloned by reverse-transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technology. The isolated cDNA is 2,421 bp in length and contains one open-reading frame (ORF) which comprises 540 amino acid residues and shows higher homology with other plant $Na^+/H^+$ antiporters. ZjNHX1 could partially complement the salt-sensitive phenotypes of ${Delta}nhx1$ and ${Delta}ena1$-$4{Delta}nhx1$ yeast mutants in the presence of NaCl, KCl, and LiCl. The expression of ZjNHX1 in Z. japonica increased after NaCl treatment and this result accords with that of $Na^+$ contents determination under the same treatment. These results implied that ZjNHX1 functions as a vacuolar $Na^+/H^+$ antiporter and plays an important role in salt tolerance and ion homeostasis in Z. japonica. |
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| 키워드 |
| Ion homeostasis;$Na^+/H^+$ antiporter;Salt tolerance;Zoysia japonica; |
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Journal of Plant Biology / v.53, no.4, 2010년, pp.251-258
한국식물학회
ISSN : 1226-9239
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO201020733090334)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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