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한국자원식물학회 / v.1, no.1, 1998년, pp.33-37 
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( Characterization of Carotenoid Biosynthetic Pathway Using Viviparous Mutant Embryos in Maize ( Zea mays L. ) ) |
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| 초 록 |
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| Carotenoid compounds in embryos of wild-type(WT) and viviparous mutants of maize(Zea mays L.) were analyzed using high performance liquid ehromatography (HPLC) with a photodiode array detector. Zeaxanthin accumulates in WT embryos as the major carotenoid. Phytoene accumulates in vp2 and vp5. Phytofluene in w3 and ${xi}$-carotene in the vp9 mutant embryos. This indicates that the vp2 and vp5 mutants impair phytoene desaturase from 15-cis-phytoene to 15-cis-phytofluene. The w3 mutant has neither an isomerase from 15-cis-phytofluene to all-trans-phytofuene nor phytofluene desaturase from phytofluene to ${xi}$-carotene. The vp9 mutant does not have the ${xi}$-carotene desaturase from ${xi}$-carotene to lycopene. Our analysis shows that the terminal carotenoid. ${gamma}$-carotene(${eta},{Psi}$-carotene), accumulates in the vp7 mutant embryos. The ${varepsilon}$-carotene(${varepsilon},{varepsilon}$-carotene), a product of ${delta}$-carotene(${varepsilon},{Psi}$-carotene) in some plants, however, has not been found in maize embryos. The vp7 mutant impairs a cyclization step from ${gamma}$-carotene to both ${eta}$-carotene and ${alpha}$-carotene. We suggest that monocyclic ${gamma}$-carotene is the sole precursor of both bicyclic ${eta}$-carotene(${eta},{eta}$-carotene) and ${alpha}$-carotene(${eta},{varepsilon}$-carotene) in maize. |
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| 키워드 |
| maize embryo;carotenoids;viviparous mutants;biosynthetic pathway; |
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Plant Resources / v.1, no.1, 1998년, pp.33-37
한국자원식물학회
ISSN : 1226-8771
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199811920279485)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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