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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.3, no.3, 2000³â, pp.173-178

( Differentiation and Detection of Phytoplasma using PCR from Diseased Plant in Korea )
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This test checked jujube witches'-broom disease, sumac witches'-broom disease, paulonia witches'- broom disease, and mulberry dwarf disease whether or not they were infected by phytoplasma, using universal and specific primers. Upon treatment of DNA amplified by PCR of phytoplasma with Alu I , Hpa II and Sat I restricted enzymes, distinction of phytoplasmas was possible. Particularly, phytoplasma of each host was distinguishable by treatment of Hpa II restricted enzyme. Meanwhile, analysis of restricted enzymes of jujube witches'-broom disease showed a higher infectivity of phytoplasmas of two origins. There were a lot of relations between jujube witches'-broom disease and sumac witches'-broom disease, and between paulonia witches'-broom disease and mulberry dwarf disease.
 
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Phytoplsama;PCR;RFLP;witches¡Ç-broom disease;
 
Plant Resources / v.3, no.3, 2000³â, pp.173-178
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ISSN : 1226-8771
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200011921401579)
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