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한국자원식물학회 / v.3, no.3, 2000년, pp.184-193 
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( QTL Analysis of Soybean Seed Weight Using RAPD and SSR Markers ) |
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| 초 록 |
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| Soybean [Glycine max (L.) Merr.] seed weight is a important trait in cultivar development. Objective of this study was to identify and confirm quantitative trait loci (QTLs) for seed weight variation in the F2 and F2:3 generations. QTLs for seed weight were identified in F2 and F2:3 generations using interval mapping (MapMaker/QTL) and single-factor analysis of variance (ANOVA). In the F2 plant generation (i.e., F3 seed), three markers, OPL9a, OPM7a, and OPAC12 were significantly (P<0.01) associated with seed weight QTLs. In the F2:3 plant row generation (i.e., F4 seed), five markers, OPA9a, OPG19, OPL9b, OPP11, and Sat_085 were significantly (P<0.01) associated with seed weight QTLs. Two markers, OPL9a and OPL9b were significantly (P<0.05) associated with seed weight QTLs in both generations. Two QTLs on USDA soybean linkage group C1 and R were identified in both F2 and F2:3 generations using interval mapping. The linkage group C1 QTL explained 16% of the variation in seed weight in both generations, and the linkage group R QTL explained 39% and 41% of the variation for F2 and F2:3 generation, respectively. The linkage group C2 QTL identified in F2:3 generation explained 14.9% of variation. Linkage groups C1, C2 and R had previously been identified as harbouring seed size QTLs. The consistency of QTLs across generations and populations indicates that marker-assisted selection is possible in a soybean breeding program. |
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| 키워드 |
| RAPD;SSR;RFLP;QTL;Soybean seed size; |
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Plant Resources / v.3, no.3, 2000년, pp.184-193
한국자원식물학회
ISSN : 1226-8771
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200011921401598)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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