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한국자원식물학회 / v.4, no.1, 2001년, pp.36-40 
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( Production of Transgenic Petunia hybrida cv. Rosanpion Using Agrobacterium-mediated Transformation ) |
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| 초 록 |
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| Transgenic Petunia hybrida cv. Rosanpion was produced by Agrobactepium tumefaciens LBA4404 harboring a binary vector pBI 121 containing $eta$-glucuronidase (gus) and neomycin phosphotransferase (nptII). For genetic transformation, leaf discs were precultured on MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BA (MNB) for 2 days and cocultured for 15 mins with A. tumefaciens. For selection of transformant, leaf discs were transferred to fresh MNB containing 50 mg/L kanamycin and 500 mg/L cefotaxime. Eighteen plants were regenerated and four were confirmed by PCR for detection of gus and nptII gene integrated into the nuclear genome of petunia ‘Rosanpion’. Using this transformation system, we expect that transgenic petunia ‘Rosanpion’ incorporating a useful gene can be produced. |
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| 키워드 |
| Petunia;Agrobacterium tumefaciens;${eta}$-glucuronidase (gus);neomycin phosphotransferase (nptII);transformation;PCR.; |
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Plant Resources / v.4, no.1, 2001년, pp.36-40
한국자원식물학회
ISSN : 1226-8771
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200111921401732)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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