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한국자원식물학회 / v.6, no.1, 2003년, pp.81-88 
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( Chiral Separation of ($pm$)-Higenamine by Capillary Electophoresis ) |
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| 초 록 |
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| Higenamine [1-(4-hydroxy-6, 7-dihydroxy-l, 2, 3, 4-tetrahydroisoquinoline) is a cardiotonic constituent of Aconiti tuber, one of the most widely prescribed oriental medicines. S-(-)higenamine was reported to have a stronger cardiotonic activity than R-(+)-higenamine and known as a central intermediate in the biosynthesis of various benzyl isoquionoline alkaloids in plants. The separation of higenamine enantiomers has been accomplished with capillary electrophoresis using cyclodextrins (CDs) as chiral selectors. Good resolution of this enantiomers was obtained using a 50 mM sodium phosphate buffer containing hydroxypropyl $eta$-CDs using 27 cm fused silica capillary (50${mu}{ extrm}{m}$ i.d., 20 cm to detector) at 25 $^{circ}C$. With the electric field of 340 V/cm, the separation time of higenamine enantiomers was less than 6 min. Under this optimum conditions, the relative standard deviations of migration time and peak area were less than 1.6% and 3.2%. A 512-channel diode array detector was confirmed for the higenamine. The detection limits (S/N = 3) of these enantiomers are $1.5mutextrm{m}$/mL. We confirmed the chiral form of higenamine in medicinal plants. |
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| 키워드 |
| Capillary electrophoresis;Higenamine enantiomers;cycoldextrin; |
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Plant Resources / v.6, no.1, 2003년, pp.81-88
한국자원식물학회
ISSN : 1226-8771
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200311921974829)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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