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한국자원식물학회 / v.7, no.3, 2004년, pp.163-169 
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( Isolation and Transcriptional Expression of CuZn Superoxide Dismutase from Codonopsis lanceolata ) |
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| 초 록 |
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| To investigate the defense mechanism against the abiotic stress, a cDNA clone encoding a CuZn superoxide dismutase (CuZnSOD) protein was isolated from a cDNA library prepared from tabroot mRNAs of Codonopsis lanceolata. The eDNA, designated ClSODCc, is 799 nucleotides long and has an open reading frame of 459 bp with a deduced amino acid sequence of 152 residues. The deduced amino acid sequence of ClSODCc matched to the previously reported CuZnSODs. Consensus amino acid residues (His-45, -47, -62, -70, -79, -119 and Asp-82) were involved in Cu-, Cu/Zn-, and Zn- binding ligands. The deduced amino acid sequence of ClSODCc showed high homologies (82％-86％) regardless of species. Expression of ClSODCc by oxidative stress was increased up to 1 h after treatment and declined gradually. Much earlier and stronger expression of ClSODCc was observed in the cold stress treatment. |
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| 키워드 |
| abiotic stress;Codonopsis lanceolata;RT-PCR;superoxide dismutase; |
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Plant Resources / v.7, no.3, 2004년, pp.163-169
한국자원식물학회
ISSN : 1226-8771
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200414714198218)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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