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한국자원식물학회 / v.7, no.3, 2004년, pp.174-180 
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( Isolation and Characterization of Calmodulin 2 (CICAM2) Gene from Codonopsis lanceolata ) |
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| 초 록 |
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| Calmodulin, a $Ca^{2+}$-binding protein, has no enzyme activity. It combines with $Ca^{2+}$ and makes variable proteins to an active form. Calmodulin 2 is a ubiquitous protein in plants. To investigate the defense mechanism against various stresses, a clone encoding a calmodulin 2 protein was isolated from a cDNA library prepared from taproot mRNAs of Codonopsis lanceolata. The cDNA, designated CICAM2, is 719 nucleotides long and has an open reading frame of 450 bp with a deduced amino acid sequence of 149 residues. The deduced amino acid sequence of CICAM2 showed a high similarity with calmodulins of P. x hybrida (P27163) 97%, N. tabacum (BAB61908) 97%, S. tuberosum (AAA74405) 96%, Z. mays (CAA74307) 92%, C. richardii (AF510075) 93%, M. truncatula (AAM81203) 91%, and G. max (P62163) 91%. The transcriptional expression of the CICAM2 gene, was gradually increased by the CaCl$_2$ treatment. Whereas its expression And it was gradually decreased in the cold stress treatment.ent. |
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| 키워드 |
| abiotic stress;Codonopsis lanceolata;RT-PCR;calmodulin 2; |
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Plant Resources / v.7, no.3, 2004년, pp.174-180
한국자원식물학회
ISSN : 1226-8771
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200414714198338)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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