|
|
|
한국자원식물학회 / v.7, no.3, 2004년, pp.200-205 
|
( Elimination of SPFMV from Virus-infected Sweet Potato Plants through Apical Meristem Culture ) |
| ;;;; ;;;;
|
|
|
 |
|
| |
| 초 록 |
|
|
| Sweet potato infected with a viral disease (SPFMV) showed irregular chlorotic patterns, so called feathering associated with faint or distinct ring spots that have purple-pigmented borders. SPFMV was eliminated from sweet potato plants using meristem tip culture. MS medium supplemented with BAP (2mg/L) and NAA (0.05 mg/L) was used for shoot proliferation and 1/2 MS medium for rooting of the plants. Highest percentage of regenerated plants (60%) was obtained from the optimum size (0.3-0.5mm) meristem tips. Of these, 60% plants were found negative for SPFMV by RT-PCR. Virus detection by RT-PCR was found to be a reliable method. Meristem-tip culture to produce SPFMV-free quality sweet potato and virus detection by RT-PCR is an efficient, time saving and reliable method for production of SPFMV-free tissue culture raised plants. |
| |
| 키워드 |
| Sweet potato;meristem tip culture;shoot proliferation;SPFMV;RT-PCR.; |
| |
|
|
|
|
Plant Resources / v.7, no.3, 2004년, pp.200-205
한국자원식물학회
ISSN : 1226-8771
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200414714198578)
언어 : 영어 |
|
| 논문 제공 : KISTI 한국과학기술정보연구원 |
|
|
|
|
|
|
