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한국자원식물학회 / v.8, no.3, 2005년, pp.286-292 
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( Plant Regeneration from Turnip (Brassica rapa ssp. rapifera) Organs ) |
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| 초 록 |
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| Shoot induction system was developed in the recalcitrant plant species, Brassica rapa ssp. rapifera by using optimum selection of profit organ, phytohormone combination, seedling age and kind of culture container. Out of in vitro cultured leaf segment, petiole, hypocotyl, and cotyledon with petiole, only cotyledon with petiole derived from 4 day-old seedlings induced multiple shoot. The optimum combination of auxin and cytokinin for the multiple shoot induction was MS medium containing 5mg/L BA and 0.5mg/L NAA. The major factors for multiple shoot propagation were part of plant organ, age of seedling, and ratio of auxin and cytokinin. In addition, shoot regeneration was promoted in the 100ml Erlenmeyer flask compared with the $90mm{ imes}20mm$ Petri-dish. The induced shoots formed roots easy on MS medium containing 0.1mg/L IBA and the whole plants were successfully cultivated in soil. |
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| 키워드 |
| BA ($N^6$-benzyladenine);Brassica;cotyledon;hypocotyl;NAA (${alpha}$-naphthalene acetic acid);multiple shoot;petiole; |
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Plant Resources / v.8, no.3, 2005년, pp.286-292
한국자원식물학회
ISSN : 1226-8771
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200517033413948)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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