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한국자원식물학회 / v.2, no.2, 1999년, pp.69-74 
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( Capsid Protein Gene Sequence Analysis and Development of Diagnostic Method by RT-PCR of Barley Yellow Mosaic Virus ) |
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| 초 록 |
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| A rapid and sensitive assay for specific detection and identification of barley yellow mosaic virus(BaYMV) was set up using the reverse transcriptase polymerase chain reaction(RT-PCR). A couple of primers was select to discriminate the viruses. PCR fragments of BaYMV(ca.0.9 kb) were obtained by using the method designed for BaYMV capsid protein. RT-PCR fragments were cloned with vector pT7 Blue and the resulting clones were sequenced. Capsid protein of BaYMV consisted of 297 amino acids and 891 nucleotides. The capsid protein sequence of BaYMV showed that 98% of nucleotides and 99% of amino acids homology. |
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| 키워드 |
| BaYMV;RT-PCR;capsid protein;and sequence; |
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Plant Resources / v.2, no.2, 1999년, pp.69-74
한국자원식물학회
ISSN : 1226-8771
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199911921398753)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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