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한국자원식물학회 / v.1, no.2, 1998년, pp.113-113 
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Interrelation between N and S Nutrition on Accumulation of Storage Protein in Soybean Seed
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| Peak,Nam Chon;Richard Shibles; 서울대학교 Div. of Plant Sci.;Dept. of Agronomy, Iowa State Univ.;
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| 초 록 |
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| Soybean is an important crop because its seed has very high protein relative to others. The quality of soy protein is limited by the concentration of the sulfur-containing amino acids in the amino acid profile. Among the supply of various forms of 0.4mM sulfur as S nutrition during seed fill, only 0.4mN L-methionine can inhibit β-subunit synthesis completely and produce the highest glycinin-containing seeds. Compared to 0.4mN sulfate control, seeds supplied by 0.4mM L-methionine have lower α-, no β-subunit, and highly increased glycinin without altering total protein concentration Supply of 0.2mM cystine (0.4mMS) did not affect the accumulative pattern of seed storage protein (SSP) subunits. In the supply of L-methionine, 0.2mM treatment showed higher glycinin in seeds but 0.05mM resulted in lower glycinin than the sulfate control. The relative abundance of α'-subunit was not altered by any N or S nutrition. Under 5mM nitrogen, protein concentration was increased about 3-5% by substituting ammonia for nitrate during seed fill independent of S nutrition. This increase resulted in the only increase of 7S protein, mainly β-subunit. Our data suggest that the regulatory system of SSP genes responds to the balance between N and S assimilates supplied from mother plant, and controls the differential synthesis of their subunits for the maximum protein accumulation in developing soybean seed. |
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| 키워드 |
| soybean;seed storage protein;glycinin;β-Conglycinin;sulfate;L-Cystine;L-Methionine;nitrate;urea; |
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한국자원식물학회지 / v.1, no.2, 1998년, pp.113-113
한국자원식물학회
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199810102439735)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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