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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.23, no.1, 2010³â, pp.38-46
ÀÚ»ý ¾çÄ¡½Ä¹° Áö»óºÎ¿Í ±Ù°æ ¸Þź¿Ã ÃßÃâ¹°ÀÇ Ç×»êÈ­ È¿°ú
( Antioxidant Effects of the Methanol Extracts Obtained from Aerial Part and Rhizomes of Ferns Native to Korea )
½Å¼Ò¸²;ÀÌöÈñ; ÃæºÏ´ëÇб³ ÀÀ¿ë»ý¸íȯ°æÇкΠ¿ø¿¹°úÇаú;ÃæºÏ´ëÇб³ ÀÀ¿ë»ý¸íȯ°æÇкΠ¿ø¿¹°úÇаú;
 
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¸Þź¿ÃÀ» ¿ë¸Å·Î 12Á¾ ¾çÄ¡½Ä¹°ÀÇ Áö»óºÎ¿Í ±Ù°æÀÇ °ÇÁ¶½Ã·á¸¦ ÃÊÀ½ÆÄÃßÃâÇÏ¿© Æä³î¼º ¹°Áú ÇÔ·® ¹× DPPH¿Í ABTS radical ¼Ò°Å´ÉÀ» ÃøÁ¤ÇÏ¿´´Ù. ÃÑ Æú¸®Æä³îÀÇ ÇÔ·®Àº °ÇÁ¶½Ã·á 1 g¿¡ $2.793{sim}140.014;mg{cdot}g^{-1}$, »ý½Ã·á 1 g¿¡ $0.779{sim}49.579;mg{cdot}g^{-1}$À¸·Î ´Ù¾çÇÏ°Ô ³ªÅ¸³µÀ¸¸ç, ³ËÁÙ°í »ç¸®¿Í ´õºÎ»ìÀ̰í»ç¸®ÀÇ Áö»óºÎ¿¡¼­ ÃÑ Æú¸®Æä³î ÇÔ·®ÀÌ ³ô¾Ò´Ù. ÃÑ Çö󺸳ëÀ̵å ÇÔ·®Àº °ÇÁ¶½Ã·á 1 g¿¡ $2.559{sim}34.909;mg{cdot}g^{-1}$, »ý½Ã·á 1g¿¡ $0.714{sim}11.487;mg{cdot}g^{-1}$À¸·Î ³ªÅ¸³µ´Ù. °ÇÁ¶½Ã·á Áß¿¡´Â °üÁß Áö»óºÎ, »ý½Ã·á Áß¿¡´Â ´õ ºÎ»ìÀ̰í»ç¸®ÀÇ Áö»óºÎ¿¡¼­ ÃÑ Çö󺸳ëÀ̵å ÇÔ·®ÀÌ °¡Àå ³ô¾Ò´Ù. ÃÑ Æú¸®Æä³îÀº Áö»óºÎº¸´Ù ±Ù°æ¿¡ ¸¹ÀÌ ÇÔÀ¯µÈ °æÇâÀ» º¸¿´À¸³ª, ÃÑ Çö󺸳ëÀ̵å´Â Áö»óºÎ¿¡ ¸¹¾Ò´Ù. DPPH¿Í ABTS radical ¼Ò°Å´ÉÀº Áö»óºÎº¸´Ù ±Ù°æ ÃßÃâ¹°¿¡¼­ ³ô°Ô ³ªÅ¸³µ´Ù. ÃÑ Æú¸®Æä³îÀº Áö»óºÎº¸´Ù ±Ù°æ¿¡ ¸¹ÀÌ ÇÔÀ¯µÈ °æÇâÀ» º¸¿´À¸³ª, ÃÑ Çö󺸳ëÀ̵å´Â Áö»óºÎ¿¡¼­ ÇÔ·®ÀÌ ¸¹¾Ò´Ù. DPPH¿Í ABTS radical ¼Ò°Å´ÉÀº Áö»óºÎº¸´Ù ±Ù°æ ÃßÃâ¹°¿¡¼­ ³ô°Ô ³ªÅ¸³µ´Ù. DPPH radical ¼Ò°Å´É°ú ABTS radical ¼Ò°Å´É ¸ðµÎ ³ªµµÈ÷ÃÊ¹Ì ±Ù°æ ÃßÃâ¹°¿¡¼­ °¡Àå ¿ì¼öÇÏ¿´´Ù. ±×·¯³ª ½Ã·áÀÇ ¼öºÐÇÔ·®°ú ÃßÃâ¼öÀ²À» °í·ÁÇÏ¿© $RC_{50}$À» ³ªÅ¸³»´Âµ¥ ÇÊ¿äÇÑ »ý½Ã·áÀÇ ¾çÀ» °è»êÇÑ °á°ú, ³ªµµÈ÷ÃÊ¹Ì ±Ù°æº¸´Ù´Â ´õºÎ»ìÀ̰í»ç¸® Áö»óºÎ°¡ DPPH¿Í ABTS radical ¼Ò°Å¿ë ½Ä¹°¼ÒÀç·Î ÀûÇÕÇÏ¿´´Ù. ¾çÄ¡½Ä¹°ÀÇ ÃßÃâ¹°Àº ƯÈ÷ ABTS radical ¼Ò°Å´ÉÀÌ ¸Å¿ì ¿ì¼öÇÏ¿´À¸¸ç, °¡Áö°íºñ°í»ç¸® ÃßÃâ¹°À» Á¦¿ÜÇÑ ´ëºÎºÐÀÇ ½Ã·á°¡ ascorbic acid ¶Ç´Â BHT¿Í À¯»çÇÑ ¼Ò°ÅȰ¼ºÀ» º¸¿´´Ù.
Phenolic compound contents and scavenging activity on DPPH and ABTS radicals in twelve fern species were studied by analysing ultrasonification extraction of dried aerial parts and rhizomes using methanol solvent. Total polyphenol content ranged from 2.97 to 140.1 mg per 1 g dried sample and 0.80 to $49.58;mg{cdot}g^{-1}$ fresh sample. Highest polyphenol content was obtained with aerial part of Davallia mariesii and Polystichum lepidocaulon. Total flavonoid content of dried sample was $2.56{sim}34.91;mg{cdot}g^{-1}$ and fresh sample $0.71{sim}11.49;mg{cdot}g^{-1}$. Higher flavonoids were obtained with dried aerial part of Dryopteris crassirhizoma, but with fresh aerial part of P. lepidocaulon. In general, total polyphenol content was higher in rhizomes, except in case of total flavonoid. Scavenging effects on DPPH and ABTS radicals was higher with rhizome extracts compared to aerial parts. The rhizome of Polystichum polyblepharum showed highest effects on both radicals. However, the amount of fresh sample for $RC_{50}$ with consideration of water content and extraction yield. P. lepidocaulon aerial part was more advantageous than P. polyblepharum rhizome. All the fern extracts except for the Coniogramme japonica extract demonstrated superior scavenging effects on ABTS radicals, being similar activity of ascorbic acid and BHT.
 
Ű¿öµå
ABTS radical;DPPH radical;polyphenol;flavonoid;sonication;ultrasonic wave;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.23, no.1, 2010³â, pp.38-46
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO201021147395664)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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