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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.23, no.1, 2010³â, pp.25-30
¾Ö±âÀå´ë AtSIZ3 º¯ÀÌÇüÀÇ ¿Âµµ ¹× °ÇÁ¶ ½ºÆ®·¹½º¿¡ ´ëÇÑ ¹ÝÀÀ°ú À¯ÀüÀÚ ¹ßÇö
( Gene Expression and Response of Arabidopsis AtSIZ3 Mutants to Temperature and Drought Stress )
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¾Ö±âÀå´ëÀÇ AtSIZ3(At1g08910) À¯ÀüÀÚ¿¡ T-DNA¸¦ »ðÀÔÇÑ ¼¼ Á¾·ùÀÇ º¯ÀÌÇü¿¡ Àú¿Â($4^{circ}C$), °í¿Â($37^{circ}C$) ¹× °ÇÁ¶½ºÆ®·¹½º¸¦ ó¸®ÇÏ¿© À¯¹¦ÀÇ »ýÀå¹ÝÀÀ°ú À¯ÀüÀÚ ¹ßÇöÀ» Á¶»çÇÏ¿´´Ù. Àú¿Â°ú °í¿Â󸮿¡ ÀÇÇØ¼­´Â ¾ß»ýÇü°ú º¯ÀÌÇü°£¿¡ À¯¹¦»ýÀå¿¡ À¯ÀÇÇÑ Â÷À̸¦ º¸ÀÌÁö ¾Ê¾Ò´Ù. ¾ß»ýÇü°ú º¯ÀÌÇü ½Ä¹°Ã¼¿¡ 10Àϰ£ÀÇ °ÇÁ¶½ºÆ®·¹½º¸¦ ó¸®ÇÏ¸é ¾ß»ýÇüÀº Àç °ü¼ö¿¡ ÀÇÇØ ¸ðµç ½Ä¹°Ã¼°¡ Àç»ýÇÏ¿´À¸³ª º¯ÀÌÇüÀº ¸ðµÎ °í»çÇÏ¿´°í, 10Àϰ£ÀÇ °ÇÁ¶Ã³¸®·Î º¯ÀÌÇüÀº ¾ß»ýÇü¿¡ ºñÇØ À¯¹¦»ýÀåÀÌ Æò±Õ 62.9%°¡ ¾ïÁ¦µÇ´Â °ÍÀ¸·Î ³ªÅ¸³µ´Ù. ¾ß»ýÇü¿¡¼­ AtSIZ3 À¯ÀüÀÚ´Â $4^{circ}C$ÀÇ Àú¿Â󸮿¡¼­ ¹«Ã³¸®¸¦ º¸´Ù 20%Á¤µµ ¹ßÇöÀÌ °¨¼ÒÇÏ´Â ¹Ý¸é, $37^{circ}C$ °í¿Â󸮿¡¼­´Â 3.7¹è, °ÇÁ¶Ã³¸®¿¡¼­´Â 4.5¹è°¡ Áõ°¡ÇÏ¿´´Ù. ÀÌ °á°ú·Î º¸¾Æ AtSIZ3 À¯ÀüÀÚ´Â ½Ä¹°ÀÇ °ÇÁ¶³»¼º°ú ¹ÐÁ¢ÇÑ ¿¬°üÀÌ ÀÖÀ» °ÍÀ¸·Î ÆÇ´ÜµÈ´Ù.
This study was carried out to understand the effect of low temperature($4^{circ}C$), heat shock($37^{circ}C$) and drought stresses on the growth and gene expression of Arabidopsis ATSIZ3(at1g08910) mutants. The seedling growth of SIZ3-mutants were markedly inhibited by the treatment of heat shock or chilling stresses. However, there was no significant differences between wild type and SIZ3-mutants in seeding fresh weight. As compared to wild type plants, SIZ3-mutants showed 63.9% inhibition of seedling fresh weight by the treatment of 10 days drought stress, suggesting that SIZ3 is involved in the resistance of Arabidopsis to drought stress. Base on RT-PCR analysis, expression of SIZ3 mRNA in the wild type showed 20% inhibition by chilling stress, 3.7 and 4.5 fold increase by the treatment of heat shock or drought stresses, respectively.
 
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Chilling;Heat shock;Drought stress;Mutants;Gene expressing;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.23, no.1, 2010³â, pp.25-30
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO201021147395660)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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