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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.23, no.1, 2010³â, pp.14-18
Agrobacterium rhizogenes strainsÀÌ ¹èÃÊÇâ ¸ð»ó±Ù À¯µµ¿Í Rosmarinic acid »ý»ê¿¡ ¹ÌÄ¡´Â ¿µÇâ
( Influence of Different Strains of Agrobacterium rhizogenes on Hairy Root Induction and Rosmarinic Acid Production in Agastache rugosa Kuntze )
±èÁ¾¼¼;¿ÀÀºÁ¤;À̼÷¿µ; Á¶¼±´ëÇб³ »ý¹°Çаú;Á¶¼±´ëÇб³ »ý¹°Çаú;Á¶¼±´ëÇб³ ³ëÀα¸°­ÁúȯÁ¦¾î¿¬±¸¼¾ÅÍ;
 
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rosmarinic acid »ý»ê¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» ¾Ë¾Æº¸±â À§ÇÏ¿© 5°èÅëÀÇ strain (13333, 15834, R1000, R1200, R1601)À» ÀÌ¿ëÇÏ¿© Á¶»çÇÑ °á°ú A. rhizogenes R1601ÀÌ °¡Àå ³ôÀº 72.9%ÀÇ ¸ð»ó±Ù À¯µµÀ²À» º¸¿´´Ù. ¸ð»ó±Ù À¯µµ½Ã ¸ð»ó±Ù ¹ß»ý ¼ö¿Í ±æÀÌ ½ÅÀå¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» Á¶»çÇÑ °á°úµµ A. rhizogenes R1601À¸·Î °¨¿°½ÃŲ ÀÙ Á¶Á÷¿¡¼­ Æò±Õ 5.4°³ÀÇ ¸ð»ó±ÙÀÌ ¹ß»ýÇÏ¿´À¸¸ç, Æò±Õ 2.0 cmÀÇ ±æÀÌ ½ÅÀåÀ¸·Î °¡Àå ÁÁÀº °á°ú¸¦ ³ªÅ¸³Â´Ù. ´Ù¼¸ °èÅëÀÇ A. rhizogenes°¨¿°À¸·ÎºÎÅÍ À¯µµµÈ °¢°¢ÀÇ ¸ð»ó±ÙÀÇ »ýÀ°Àº ¼­·¯ ´Ù¸¥ ¾ç»óÀ» º¸¿´À¸³ª °æÇâÀº ¸ð»ó±ÙÀ¯µµ °á°ú¿Í À¯»çÇÏ¿´´Ù. A. rhizogenes R1601 °¨¿°À¸·Î À¯µµµÈ ¸ð»ó±ÙÀÇ »ýÀ°Àº ¹è¾ç 2ÁÖÈÄ °Ç¹°ÁßÀ» Á¶»çÇÑ °á°ú 13.5 g/l·Î ´Ù¸¥ °èÅë¿¡¼­ À¯µµµÈ ¸ð»ó±Ù º¸´Ù »ýÀ°ÀÌ ¾çÈ£ÇÏ¿´À¸¸ç, Rosmarinic acid »ý»ê·®µµ 22.6 mg/g D.W.·Î ´Ù¸¥ °èÅë¿¡ ºñÇÏ¿© °¡Àå ³ô°Ô ³ªÅ¸³µ´Ù. A. rhizogenes R1601ÀÌ ¹èÃÊÇâ ¸ð»ó±Ù À¯µµ, »ýÀ°°ú rosmarinic acid »ý»ê¿¡ °¡Àå ÁÁÀº ÀûÇÕÇÑ °èÅëÀ̾úÀ½À» ¾Ë ¼ö ÀÖ¾ú´Ù.
Rosmarinic acid, an ester of caffeic acid with 3,4-dihydroxyphenyl lactic acid, is one of the main active constituents of Agastache rugosa Kuntze and has an astringent property, antioxidant capacity, anti-inflammatory activity, antimutagenic ability, antimicrobial capacity, and an antiviral property. Five different strains of Agrobacterium rhizogenes differed in their ability to induce Korean mint (Agastache rugosa Kuntze) hairy roots and also showed varying effects on the growth and rosmarinic acid production in hairy root cultures. A. rhizogenes R1601 is the most effective strain for the induction (72.90%), growth (13.50 g/l) and rosmarinic acid production (22.60 mg/g) in hairy root of Korean mint. Our results demonstrate that use of suitable strains of A. rhizogenes may allow study of the regulation of rosmarinic acid biosynthesis in hairy root cultures of Agastache rugosa.
 
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Agastache rugosa Kuntze;Agrobacterium rhizogenes;Hairy root;Rosmarinic Acid;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.23, no.1, 2010³â, pp.14-18
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO201021147395656)
¾ð¾î : Çѱ¹¾î
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