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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.21, no.5, 2008³â, pp.388-394
ºÐÀÚÀ°Á¾±â¹ý¿¡ ÀÇÇØ ¼±¹ßµÈ ÇüÁúÀüȯ º­ °èÅëÀÇ ÀÛ¹°ÇÐÀû Ư¼º
( Characteristics of Agronomy Traits to Transgenic Rice Selected by Molecular Breeding Method )
ÀÌÇö¼÷;°­Çö±¸;¹Ú¿µÈñ;Á¤Èñ¿µ;±èâ±æ;ÇÑÁõ¼ú;¼ÕÀç±Ù;±è°æ¹Î;¹Ú±Ôȯ; °æºÏ´ëÇб³ ÀÀ¿ë»ý¸í°úÇкÎ;°æºÏ´ëÇб³ ½Ä¹°ÀÚ¿øÇаú;°æºÏ´ëÇб³ ÀÀ¿ë»ý¸í°úÇкÎ;°æºÏ´ëÇб³ ÀÀ¿ë»ý¸í°úÇкÎ;°æºÏ´ëÇб³ ȯ°æ¿ø¿¹Çаú;±¹¸³¿ø¿¹Æ¯ÀÛ°úÇпø;°æºÏ´ëÇб³ ÀÀ¿ë»ý¸í°úÇкÎ;°æºÏ´ëÇб³ ȯ°æ¿ø¿¹Çаú;°æºÏ´ëÇб³ ½Ä¹°ÀÚ¿øÇаú;
 
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Arabidopsis thaliana $Ca^{2+}/H^+$ ¼ö¼Ûü À¯ÀüÀÚÀÎ CAX 1(cation exchanger 1)ÀÌ µµÀÔµÈ º­ ÇüÁúÀüȯü¿¡ ´ëÇÑ ¿¬±¸°á°ú¸¦ ¿ä¾àÇÏ¸é ´ÙÀ½°ú °°´Ù. Agrobacterium ÀÌ¿ë ÇüÁúÀüȯ CAX 1 º­ $T_5$ ¼¼´ë¸¦ ´ë»óÀ¸·Î CAX 1¹êµå°¡ µµÀԵǾúÀ½À» È®ÀÎÇÏ¿´´Ù. ³ó¾÷ÀûÀΠƯ¡ÀÎ ¼öÀå, ¼ö¼ö ¹× °£ÀåÀº ¸ðǰÁ¾(ÀÏǰ)°ú À¯»çÇÏ¿´À¸¸ç, Á¾ÀÚÀÇ Ä®½· ÇÔ·®µµ ³ô¾Ò´Ù. ¹Ì¸³ÀÇ Å©±â¿Í õ¸³ÁßÀº ¸ðǰÁ¾º¸´Ù ¾à°£ Å©³ª °ÅÀÇ ÀÏÄ¡Çϰí, Àú¿ÂÀúÇ×¼º°ú µµ¿­º´¿¡ ´ëÇÑ ³»º´¼ºÀÌ ³ªÅ¸³µ´Ù. $T_5$ ¼¼´ë¿¡¼­ ÇüÁúÀÌ ¾ÈÁ¤ÀûÀ¸·Î ¹ßÇöµÇ¸é¼­ ¸ðǰÁ¾°ú À¯»çÇÏ°í ³ó¾÷Àû ÇüÁúÀÌ ¿ì¼öÇÑ 3 °èÅëÀ» ¼±¹ßÇÏ¿´´Ù. ¾ÕÀ¸·Î $T_6$ ¼¼´ë¿¡ ´ëÇÑ Æ¯¼ºÁ¶»ç¸¦ °ÅÃÄ $Ca^{2+}$ ÇÔ·®ÀÌ ¾ÈÁ¤ÀûÀ¸·Î ³ô°Ô À¯ÁöµÇ´Â °èÅëÀ» ¼±¹ßÇÏ¿© ¼¼´ëÀ°¼ºÇϸé Ä®½·°ú °ü·ÃµÈ À¯ÀüÀÚÀÇ ³»ÀçÇØ¼º ÀÛ¹°ÀÇ ±âÃÊÀÚ·á ÀÌ¿ëµÇ¾î Áú ¼ö ÀÖÀ» °ÍÀ¸·Î »ç·áµÈ´Ù.
This study was carried out to develop new cultivars using the $T_5$ generation of transformed rice by PCR analysis with DNA marker in each generation $(from;T_3;to;T_5)$. In the previous study, we successfully developed the transgenic rice plants over-expressing the Arabidopsis $H^+/Ca^{2+}$ antiporter CAX 1 (accession no. U57411) gene. The calcium concentration in brown rice of transgenic plants was higher than that of donor plants, Iipum, and was selected 3 lines out of 25 lines at cultured GMO field. The major agronomic traits such as culm length, panicle length and panicle number of 3 lines at transgenic plants $(T_5)$ were similar to wild type. Also these lines appeared to have disease resistance to rice blast, cold resistance as compared with donor types. The grain shape was similar to donor plant, however, the 1000 grain weight of brown rice was different from transgenic plants. These finding would be used for basic data of new variety registration.
 
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Rice;Molecular breeding;CAX 1;Disease resistance;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.21, no.5, 2008³â, pp.388-394
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200800557084055)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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