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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.21, no.5, 2008³â, pp.408-412
±¸¾Æ¹Ù(Psidium guajava L.) ÀÙÀÇ Ç×»êÈ­ Ȱ¼º ¹× tyrosinase ÀúÇØÈ¿°ú
( Antioxidant activities and tyrosinase inhibitory effects of guava (Psidium guajava L.) leaf )
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º» ¿¬±¸´Â ±â´É¼º¼ÒÀç °³¹ß¿¡ ÀÌ¿ëµÉ ¼ö ÀÖ´Â À¯¿ëÇÑ ±âÃÊÀڷḦ ¾ò°íÀÚ ±¸¾Æ¹Ù ÀÙÀÇ ÃÑÆä³îÈ­ÇÕ¹°ÀÇ ÇÔ·®, Ç×»êȭȰ¼º, tyrosinase ÀúÇØÈ°¼ºÀ» ÃøÁ¤ÇÏ¿´´Ù. 1. ±¸À̹ÙÀÙ ÃßÃâ¹°ÀÇ ÃÑÆä³îÈ­ÇÕ¹°Àº 19.0(g/100g, D.W.)ÀÇ ÇÔ·®À¸·Î °­ÇÑ Ç×»êȭȰ¼ºÀ» º¸¿´´Ù(radical ¼Ò°ÅȰ¼ºÀÇ IC50´Â $102.5{mu}g/ml$, SOD À¯»çȰ¼ºÀÇ IC50´Â $49.4{mu}g/ml).$) 2. ¿ë¸ÅºÐȹ¹°ÀÇ radical ¼Ò°ÅȰ»óÀ» ÃøÁ¤ÇÑ °á°ú, $100{mu}g/ml$ÀÇ ³óµµ¿¡¼±µµ ethylacetate(G-E), butanol(G-B), ¹°ºÐȹ(G-W)Àº 50% ÀÌ»óÀÇ ³ôÀº ¼Ò°ÅȰ¼ºÀ» º¸¿´´Ù radical ¼Ò°Å Ȱ¼ºÀº G-B>G-E>G-W ¼øÀ¸·Î ³ôÀº Ȱ¼ºÀ» º¸¿´°í, °¢ ºÐȹ¹°ÀÇ ³óµµ°¡ Áõ°¡ÇÒ¼ö·Ï ¼Ò°Å´ÉÀÌ ³ô¾ÆÁö´Â °æÇâÀ» ³ªÅ¸³Â´Ù. hexaneºÐȹ(G-H)ÀÇ ¼Ò°ÅȰ¼ºÀº º¸ÀÌÁö ¾Ê¾Ò´Ù. 3. ¿ë¸ÅºÐȹº° SOD À¯»çȰ¼º¿¡ ´ëÇÑ °á°ú, $100{mu}g/ml$ÀÇ ³óµµ¿¡¼­ G-E, G-B, G-W´Â °¢°¢ 81.8, 84.7, 65.3%·Î ³ôÀº Ȱ¼ºÀ» ³ªÅ¸³ÂÀ¸¸ç, °¢ ºÐȹ¹°ÀÇ ³óµµ°¡ Áõ°¡ÇÒ¼ö·Ï SOD À¯»çȰ¼ºµµ Áõ°¡ÇÏ´Â °æÇâÀ» ³ªÅ¸³Â´Ù G-HÀÇ È°¼ºÀº º¸ÀÌÁö ¾Ê¾Ò´Ù. 4. ±¸¾Æ¹ÙÀÙÀÇ ÃßÃâ¹°°ú ¿ë¸ÅºÐȹ¹°ÀÇ tyrosinaseÀúÇØ È¿°ú¸¦ ÃøÁ¤ÇÑ °á°ú, ÃßÃâ¹° 1mg/mlÀÇ ³óµµ¿¡¼­ 60.8%ÀÇ ÀúÇØÈ¿°úÀ» º¸¿´À¸¸ç, ¿ë¸ÅºÐȹÀº G-E(65.2%), G-B(62.8%), G-W(51.6%), G-H(28.9%)ÀÇ ¼øÀ¸·Î tyrosinase ÀúÇØÈ¿°ú¸¦ º¸¿´´Ù.
This research was designed to investigate the antioxidant activities and tyrosinase inhibitory effects of guava (Psidium guajava L.) leaf. Total phenol content was obtained from guava leaf extract of 19.0 (g/100g, D.W.). The crude extract exhibited significantly antioxidant activities (IC50value $102.5{mu}g/ml$, free radical scavenging; $49.4{mu}g/ml$, SOD like activity). The crude extract of guava leaf was fractionated into four partition layers; hexane (G-H), ethyl acetate (G-E), butanol (G-B) and water (G-W) layer. The extracts of G-E, G-B, G-W showed high radical scavenging activities of over 50% at $100{mu}g/ml$. SOD like activities of G-E, G-B, G-W were revealed, as 81.8%, 84.7%, 65.3% at $100{mu}g/ml$, while those of G-H did not showed the effectively. The crude extract of guava leaf showed high tyrosinase inhibitory effect as 60.8% at 1mg/ml, the measurement of G-E, G-B, G-W were 65.2%, 62.8%, 51.6% and that of G-H was not effective. These results indicate that useful bioactive substances exist in the guava leaf extracts, especially G-E, G-B. And the guava leaf has the potential of being developed into health related products.
 
Ű¿öµå
guava (Psidium guajava L.);total phenol;antioxidant;tyrosinase;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.21, no.5, 2008³â, pp.408-412
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200800557084060)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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