|
|
|
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.21, no.2, 2008³â, pp.139-143
|
°¡Áö°ú½Ä¹°¿¡¼ Capsidiol »ýÇÕ¼º¿¡ °ü¿©ÇÏ´Â Cytochrome P450 À¯ÀüÀÚÀÇ ¹ßÇö°ú È¿¼ÒȰ¼º
( Enzyme Activity and Gene Expression of Cytochrome P450 Involved in Capsidiol Biosynthesis in Solanaceae Plants ) |
| ±Ç¼øÅÂ;; ¾Èµ¿´ëÇб³ »ý¸íÀÚ¿ø°úÇкÎ;ÆÛµà´ëÇб³ ¿ø¿¹Çаú;
|
|
|
 |
|
| |
| ÃÊ ·Ï |
| ´Ù¼¸ Á¾·ùÀÇ °¡Áö°ú ½Ä¹°À» ´ë»óÀ¸·Î elicitor¿¡ ÀÇÇØ »ýÇÕ¼º µÇ´Â capsidiolÀÇ ÇÔ·®À» ÃøÁ¤ÇÏ°í »ýÇÕ¼º¿¡ °ü·ÃµÈ P450 È¿¼ÒÀÇ È°¼º°ú °ü·Ã À¯ÀüÀÚ ¹ßÇöÀ» Á¶»çÇÏ¿´´Ù. °íÃß, ÇǸÁ ¹× ´ã¹è´Â capsidiolÀÇ »ýÇÕ¼º¿¡ °ü·ÃµÈ µÎ°³ÀÇ À¯ÀüÀÚÀÎ cyclase¿Í P450 À¯ÀüÀÚ°¡ ¼¼Æ÷³»¿¡ Á¸ÀçÇÏ¿´°í, elicitorÀÇ Ã³¸®¿¡ ÀÇÇÏ¿© À¯ÀüÀÚÀÇ ¹ßÇöÀÌ À¯µµµÇ°í È¿¼ÒÀÇ Ã˸Ű¡ ÀÌ·ç¾î Á³´Ù. °¡Áö¼¼Æ÷¿¡´Â µÎ°³ÀÇ À¯ÀüÀÚ°¡ °Ô³ð»ó¿¡ Á¸ÀçÇÏÁö¸¸ P450 À¯ÀüÀÚ´Â ¹ßÇöµÇ³ª, cyclase À¯ÀüÀÚÀÇ ¹ßÇöÀº ³ªÅ¸³ªÁö ¾Ê¾Ò´Ù. °¨ÀÚ¿¡´Â capsidiolÀÇ »ýÇÕ¼º¿¡ °ü·ÃµÈ P450 ¹× cyclase À¯ÀüÀÚ ¸ðµÎ°¡ Á¸ÀçÇÏÁö ¾ÊÀº °ÍÀ¸·Î ³ªÅ¸³µ´Ù. º» ½ÇÇè¿¡ »ç¿ëµÈ P450°ú cyclase À¯ÀüÀÚ´Â ½Ä¹°ÀÌ Á¤»ó»óÅ¿¡ ÀÖÀ» ¶§´Â ÀüÇô ¹ßÇöµÇÁö ¾ÊÀ¸³ª elicitor¿¡ ÀÇÇØ ƯÀÌÀûÀ¸·Î À¯µµ µÇ´Â Ư¡À» º¸¿´°í, ½Ä¹°Ã¼ Á¶Á÷À̳ª ±â°üº°·Î ´Ù¾çÇÑ ¹ßÇö ¾ç»óÀ» º¸¿´´Ù. |
|
| Enzyme activity and expression of cytochrome P450 gene involved in the pathway of capsidiol biosynthesis were compared in five different solanaceae plants such as red pepper, green pepper, tobacco, potato and egg plant. Base on genomic DNA and/or RT-PCR results, four solanaceae plants such as red pepper, green pepper, tobacco and egg plant possess P450 gene in the genome and specifically expressed by elicitor treatment. However, potato was appeared to have neither P450 nor cyclase gene in the genome. P450 genes did not show any expression in the plants under normal condition, but showed highly specific expression under elicitation condition in various organs and tissue such as leaf, root, stem and culture cells. |
| |
| Ű¿öµå |
| Solanaceae plant;Cytochrome P450;Cyclase;Capsidiol; |
| |
|
|
 |
|
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.21, no.2, 2008³â, pp.139-143
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200817848807543)
¾ð¾î : Çѱ¹¾î |
|
| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
|
|
|
|
|
|