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한국자원식물학회 / v.22, no.6, 2009년, pp.584-591 
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( Establishment of in vitro Root Cultures and Analysis of Secondary Metabolites in Indian Ginseng - Withania somnifera ) |
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| 초 록 |
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| Adventitious root culture was established in the Jawahar variety of Withania somnifera using MS basal medium supplemented with 0.5 (mg/l) IAA and 2.0 (mg/l) IBA. Root tips from germinated seedlings, MS0 maintained plants and adventitious roots were maintained in suspension medium (1/2 MS basal medium supplemented with 3% sucrose) for a period of 1 to 6 months. The weight gain in roots was noted and the withanolides were extracted from the dry roots using solvents petroleum ether, 50% ethanol and chloroform. The withanolides in the chloroform fractions of all root samples analyzed were compared using thin layer chromatographic analysis. Withanolide content in adventitious root sample was found to be superior compared to other roots at any given point of time during the 6month growth period.HPLC analysis of in vitro adventitious roots showed the presence of a new compound. |
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| 키워드 |
| Adventitious roots;germinated roots;MS0;withanolides;TLC; |
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한국자원식물학회지 / v.22, no.6, 2009년, pp.584-591
한국자원식물학회
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200916955022312)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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