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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.23, no.2, 2010³â, pp.123-130
°¡¾Ð¿­Ã³¸®°¡ ÅéÇ®°ú ¿ï¸ª¹Ì¿ªÃë ¾î¸°ÀÙÀÇ Ç×»êÈ­ Ȱ¼º¿¡ ¹ÌÄ¡´Â ¿µÇâ
( Influence of Applied Pressure and Heat Treatment on Antioxidant Activities of Young Leaves from Achillea alpina and Solidago virgaurea subsp. gigantea )
¿ìÁ¤Çâ;½Å¼Ò¸²;Á¤Çå»ó;ÀÌöÈñ; ÃæºÏ´ëÇб³ ¿ø¿¹°úÇаú;ÃæºÏ´ëÇб³ ¿ø¿¹°úÇаú;ÃæºÏ´ëÇб³ ½Äǰ°øÇаú;ÃæºÏ´ëÇб³ ¿ø¿¹°úÇаú;
 
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º» ¿¬±¸´Â ½Ä¿ë ¹× ¾à¿ëÀ¸·Î »ç¿ëµÇ´Â ¿ï¸ª¹Ì¿ªÃë¿Í ÅéÇ® ¾î¸°ÀÙÀÇ Ç×»êȭȰ¼º ¹× »ý¸®È°¼º¿¡ ¹ÌÄ¡´Â °¡¾Ð¿­Ã³¸®ÀÇ ¿µÇâÀ» ¾Ë¾Æº¸±â À§ÇÏ¿© ½ÃÇàÇÏ¿´´Ù. 4¿ù Ãʼø¿¡ ÃæºÏû¿ø±º ³ëÁö¿¡¼­ ¾î¸°ÀÙÀ» äÃëÇÑ Á÷ÈÄ °¡¾Ð¿­Ã³¸®($121^{circ}C$, 1.2±â¾Ð, 15ºÐ) ¶Ç´Â ¹«Ã³¸®ÇÏ¿© µ¿°á°ÇÁ¶ÇÑ ÈÄ ºÐ¼âÇÏ¿© 80% ¿¡Åº¿Ã ¿ë¸Å·Î ȯ·ù³Ã°¢ÃßÃâÇÑ ÃßÃâ¹°ÀÇ ÃÑ Æú¸®Æä³î ¹× Çö󺸳ëÀ̵åÀÇ ÇÔ·®, DPPH¿Í ABTS radical ¼Ò°Å´É ¹× ferrou ion chelating Á¤µµ¸¦ ÃøÁ¤ ÇÏ¿´´Ù. ÅéÇ®°ú ¿ï¸ª¹Ì¿ªÃëÀÇ ÃßÃâ¼öÀ²Àº °¡¾Ð¿­Ã³¸®ÇÑ ¿ï¸ª¹Ì¿ªÃë¿¡¼­ °¡Àå ³ô¾Ò°í(39.55%), °¡¾Ð¿­Ã³¸®ÇÑ ÅéÇ® ¾î¸°ÀÙ¿¡¼­ °¡Àå ³·¾Ò´Ù(28.15%). ÅéÇ®°ú ¿ï¸ª¹Ì¿ªÃë ¾î¸°ÀÙÀº °¡¾Ð¿­Ã³¸® ÈÄ DPPH radical ¼Ò°ÅȰ¼ºÀÌ ÇöÀúÈ÷ °¨¼ÒÇÏ¿©, °¢°¢ $1.506;mg{cdot}mL^{-1}$, $0.669;mg{cdot}mL^{-1}$¾¿ $RC_{50}$°ªÀÌ Áõ°¡ÇÏ¿´´Ù. ABTS radical ¼Ò°ÅȰ¼º ¶ÇÇÑ °¢°¢ $0.704;mg{cdot}mL^{-1}$, $0.421;mg{cdot}mL^{-1}$ ¾¿ $RC_{50}$°ªÀÌ Áõ°¡ÇÏ¿´´Ù. ¹Ý¸é ferrous ion chelate È¿°ú´Â °¡¾Ð¿­Ã³¸®¿¡ ÀÇÇÏ¿© Çâ»óµÇ¾úÀ¸¸ç, ÅéÇ®ÀÇ ¾î¸°ÀÙ¿¡¼­ Å« ÆøÀ¸·Î »ó½ÂÇÏ¿´´Ù. ¶ÇÇÑ °¡¾Ð¿­Ã³¸®´Â ÃÑ Æú¸®Æä³î°ú Çö󺸳ëÀ̵å ÇÔ·®À» °¨¼Ò½ÃÄ״µ¥, ƯÈ÷ ÅéÇ® ¾î¸°ÀÙ¿¡¼­ °¨¼ÒÆøÀÌ ÄÇ´Ù. µû¶ó¼­ º» ½ÇÇè¿¡¼­´Â °¡¾Ð¿­Ã³¸®´Â ¿ï¸ª¹Ì ¿ªÃë¿Í ÅéÇ® ¾î¸°ÀÙÀÇ Ferrous ion clelate È¿°ú¸¦ Çâ»ó½Ãų ¼ö ÀÖÁö¸¸, DPPH ¹× ABTS radical ¼Ò°Å´É°ú ÃÑ Æú¸®Æä³î ¹× Çö󺸳ëÀ̵åÀÇ ÇÔ·®À» °¨¼Ò½ÃŰ´Â °ÍÀ¸·Î ³ªÅ¸³µ´Ù. ¶ÇÇÑ, ÅéÇ®ÀÇ ¾î¸°ÀÙÀº ¿ï¸ª¹Ì¿ªÃë¿¡ ºñÇÏ¿© ¿­¾ÈÁ¤¼ºÀÌ ³·¾Æ ¿­Ã³¸®¿¡ ÀÇÇÑ È°¼ºÀÇ º¯È­°¡ Å« °ÍÀ¸·Î ³ªÅ¸³µ´Ù.
Present studies were conducted to investigate the effects of autoclaving on antioxidant activities of Achillea alpina and Solodago virgurea. At early April, young leaves of 2 species were collected, subjected to autoclaving ($121^{circ}C$, 1.2 atmospheric pressure, 15 minutes), freezed-dried, grinded, and extracted with 80% ethanol. The same process was repeated with unautoclaved control. Total polyphenol and flavonoid contents, scavenging activities on DPPH and ABTS radicals and ferrous ion chelating effects were analyzed. Extraction yield of autoclaved S. virgurea was 39.55% and A. alpina 28.15%. In both species, autoclaving significantly reduced scavenging activities on DPPH and ABTS radicals. On the contrary, ferrous ion chelating effects increased after autoclaving, especially in young leaves of A. alpina. Autoclaving resulted in decrease of polyphenol and flavonoid contents, especially in the A. alpina. The present experiments demonstrated that autoclaving had negative effects on antioxidant activities of A. alpina and S. virgurea, except in ferrous ion chelating effects. Young leaves of former species were unstable to heat treatment, resulting in big lose of antioxidant activity.
 
Ű¿öµå
ABTS radical;DPPH radical;ferrous ion chelating;flavonoid contents;polyphenol centents;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.23, no.2, 2010³â, pp.123-130
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO201021147394788)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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