¶óÆæÆ®¦¢Ä«Æä¦¢ºí·Î±×¦¢´õº¸±â
¾ÆÄ«µ¥¹Ì Ȩ ¸í»çƯ°­ ´ëÇבּ¸½Ç޹æ Á¶°æ½Ç¹« µ¿¿µ»ó°­ÀÇ Çѱ¹ÀÇ ÀüÅëÁ¤¿ø ÇÐȸº° ³í¹®
ÇÐȸº° ³í¹®

Çѱ¹°Ç¼³°ü¸®ÇÐȸ
Çѱ¹°ÇÃà½Ã°øÇÐȸ
Çѱ¹µµ·ÎÇÐȸ
Çѱ¹»ý¹°È¯°æÁ¶ÀýÇÐȸ
Çѱ¹»ýÅÂÇÐȸ
Çѱ¹¼öÀÚ¿øÇÐȸ
Çѱ¹½Ä¹°ÇÐȸ
Çѱ¹½Ç³»µðÀÚÀÎÇÐȸ
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
Çѱ¹ÀܵðÇÐȸ
Çѱ¹Á¶°æÇÐȸ
Çѱ¹Áö¹Ý°øÇÐȸ
Çѱ¹ÇÏõȣ¼öÇÐȸ
Çѱ¹È¯°æ»ý¹°ÇÐȸ
Çѱ¹È¯°æ»ýÅÂÇÐȸ

Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.22, no.2, 2009³â, pp.152-158
¼öȯ°æº¯È­¿¡ µû¸¥ °¥´ë¿Í ´Þ»Ñ¸®Ç®ÀÇ À¯ÀüÀû º¯ÀÌ ¹× À¯¿¬°ü°è
( Genetic variations and relationships of Phragmites japonica and P. communis according to water environment change )
±è¿ëÇö;±èÁÖȯ; ´ëÀü´ëÇб³ »ý¸í°úÇаú;´ëÀü´ëÇб³ »ý¸í°úÇаú;
 
ÃÊ ·Ï
°¥´ë¼Ó ½Ä¹° µÎ Á¾ ´Þ»Ñ¸®Ç®°ú °¥´ëÀÇ Á¾°£ À¯¿¬°ü°èºÐ¼® ¹× ¼öȯ°æº¯È­¿¡ µû¸¥ Á¾³» Áö¿ªº° À¯¿¬°ü°è¸¦ Á¶»çÇϱâÀ§ÇØ RAPD ºÐ¼®À» ¼öÇàÇÏ¿´´Ù. ÃÑ 9°³ÀÇ oligoprimer¸¦ ÀÌ¿ëÇÑ È¿¼ÒÁßÇÕ¹ÝÀÀ¿¡¼­ 300 bp¿¡¼­ 1,900 bp »çÀÌÀÇ ±¸°£¿¡¼­ 142°³ÀÇ À¯È¿ÇÑ polymorphic band¸¦ È®ÀÎÇÏ¿´´Ù. Nei-LiÀÇ genetic distance¸¦ ÀÌ¿ëÇÑ ºÐ¼®°á°ú¿¡ ÀÇÇϸé, ºñÀ¯»çµµÁö¼ö°¡ ´Þ»Ñ¸®Ç® °³Ã¼±º Á¾³»¿¡¼­´Â 0.012¿¡¼­ 0.061, °¥´ë °³Ã¼±º Á¾³»¿¡¼­´Â 0.033¿¡¼­ 0.095ÀÇ ³·Àº »óÀ̼ºÀ» ³ªÅ¸³»¾î Á¾³» Áö¿ªÁý´Ü°£¿¡´Â ³ôÀº À¯ÀüÀû À¯¿¬°ü°è¸¦ º¸¿´À¸¸ç, ´Þ»Ñ¸®Ç® °³Ã¼±º°ú °¥´ë °³Ã¼±º Á¾°£¿¡¼­´Â 0.043¿¡¼­ 0.132ÀÇ »óÀ̼ºÀ» ³ªÅ¸³»¾î ´Þ»Ñ¸®Ç®°ú °¥´ë »çÀÌÀÇ ºñÀ¯»çµµÁö¼öÀÇ Å« Â÷ÀÌ´Â º¸ÀÌÁö ¾Ê¾ÒÀ¸³ª À¯ÀüÀûÀ¸·Î °Å¸®°¡ ÀÖ´Â °ÍÀ¸·Î ³ªÅ¸³µ´Ù. RAPD °á°ú ÀÌ µÎ Á¾ »çÀÌ¿¡ ¶Ñ·ÇÇÑ Â÷À̸¦ ±¸º°ÇÒ ¼ö ÀÖ´Â genetic marker¸¦ È®ÀÎÇÏ¿´°í, UPGMA À¯ÁýºÐ¼®¿¡ ÀÇÇÏ¸é µÎ Á¾Àº ºñ½ÁÇÑ ¼öȯ°æ³¢¸® À¯¿¬°ü°è°¡ °¡±î¿î °ÍÀ¸·Î ³ªÅ¸³µ°í ´Ù¸¥ ¼öȯ°æ³¢¸®´Â À¯¿¬°ü°è°¡ ¸Õ °ÍÀ¸·Î ³ªÅ¸³µ´Ù. RAPD ºÐ¼®¹ýÀº °¥´ë¼Ó µÎ Á¾ÀÇ ºÐ·ùÇÐÀû È¥µ¿ ÇØ°á°ú µ¿½Ã¿¡, ¼öȯ°æº¯È­¿¡ µû¸¥ Á¾³» Áö¿ªº° À¯ÀüÀû À¯¿¬°ü°è È®Àο¡ ¸Å¿ì À¯¿ëÇÑ ¹æ¹ýÀÎ °ÍÀ¸·Î ³ªÅ¸³µ´Ù.
We performed RAPD analysis to investigate the inter-/intraspecific relationships and regional variations of Phragmites japonica and P. communis according to the environmental change. One hundred and fourty nine genetic effective polymorphic bands between 300 bp and 1,900 bp were marked from RAPD PCR with nine oligoprimers. From the RAPD analysis by Nei-Li's genetic distance, the dissimilarity indices among the populations of Phragmites japonica were relatively low from 0.012 to 0.061, and Phragmites communis were also low from 0.033 to 0.095. It showed the close genetic relationships among the same species populations, and both species were distinctly independent with relatively high level of dissimilarity indices (0.043 - 0.132). The obvious genetic markers to distinguish two species were confirmed and those profiles were suggested. From the UPGMA phenogram by RAPD analysis, both species showed the water environment related cluster patterns by distributional regions. RAPD analysis was useful to delimit two species taxonomically and to investigate the genetic relationships among inter-/intraspecific populations.
 
Ű¿öµå
RAPD;genetic marker;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.22, no.2, 2009³â, pp.152-158
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200916638024018)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
¸ñ·Ïº¸±â
ȸ»ç¼Ò°³ ±¤°í¾È³» ÀÌ¿ë¾à°ü °³ÀÎÁ¤º¸Ãë±Þ¹æÄ§ Ã¥ÀÓÀÇ ÇѰè¿Í ¹ýÀû°íÁö À̸ÞÀÏÁÖ¼Ò ¹«´Ü¼öÁý °ÅºÎ °í°´¼¾ÅÍ
   

ÇÏÀ§¹è³ÊÀ̵¿