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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.10, no.1, 1997³â, pp.94-99
¹èÁö, »ýÀåÁ¶Àý¹°Áú ¹× Ä¡»óÁ¶Á÷ÀÌ ÁöȲ ü¼¼Æ÷Á¶Á÷À¸·ÎºÎÅÍ ½Ä¹°Ã¼ºÐÈ­¿¡ ¹ÌÄ¡´Â ¿µÇâ
( Effects of Medium , Plant Growth Regulators , and Explant Sources on Plant Regeneration of Rehmannia glutinosa )
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1. ÁöȲÀÇ ÀÙÁ¶Á÷°ú ÁÙ±âÁ¶Á÷À» »ý»êÁ¶Àý¹°ÁúÀÌ Ã·°¡µÈ MS¹èÁö¿¡ Ä¡»óÇÏ¿´À» ¶§ Quincrac, NAA, 2.4-D¿¡¼­ º¸´Ù TDZ¿¡¼­ ´õ ¸¹Àº ½ÅÃʺÐÈ­°¡ ÀϾ´Ù. »Ñ¸®ºÐÈ­´Â ½ÅÃʺÐÈ­°¡ ÀߵǾú´ø TDZ¿¡¼­¿Í´Â ´Þ¸® NAA°¡ ÷°¡µÈ ¹èÁö¿¡¼­ ÀÙÁ¶Á÷À» Ä¡»óÇßÀ» ¶§ ºÐÈ­°¡ ¸¹ÀÌ ÀϾTEk. 2. ÁöȲÀÇ ÀÙÁ¶Áö°ú ÁÙ±âÁ¶Á÷À» $B_5$¹èÁö¿¡ Ä¡»óÇÏ¿´À» ¶§ Quinerac, NAA, 2.4-D¿¡¼­º¸´Ù TDZ¿¡¼­ ½ÅÃʺÐÈ­°¡ ¸¹ÀÌ ÀϾ´Ù. $B_5$¹èÁö¿¡¼­´Â Quincrac, NAA, 2.4-D ¸ðµÎ 0.01mg/l¿¡¼­ ºÐÈ­°¡ ÀϾ´Ù. »Ñ¸®ÀÇ ºÐÈ­¿¡¼­ $B_5$¹èÁö¿¡¼­´Â NAA, TDZ ¸ðµÎ ºÐÈ­°¡ Àß µÇ¾ú´Ù. $B_5$¹èÁö¿¡¼­´Â Quincrac°ú 2.4-D¿¡¼­µµ »Ñ¸®ºÐÈ­ÀÇ È¿À²ÀÌ ³ô¾Ò´Ù. 3. 2.4-D¿Í TDZ »ýÀåÁ¶Àý¹°ÁúÀ» Á¶ÇÕó¸®ÇÑ MS¹èÁö¿¡ ÁöȲÀÇ ÀÙ°ú ÁÙ±âÁ¶Á÷À» Ä¡»óÇÑ °á°ú ½ÅÃʼö¿Í ±æÀÌ´Â 2.4-D 0.01mg/l¿Í TDZ 0.01, 0.1, 2(${mu}M$)ÀÇ Á¶ÇÕ󸮿¡¼­ °¡Àå ¸¹Àº ½ÅÃʺÐÈ­¼ö¿Í ±æÀ̸¦ ³ªÅ¸³Â´Ù. 2.4-D¿Í TDZÀ» Á¶ÇÕó¸®ÇÑ $B_5$¹èÁö¿¡ ÁöȲÀÇ ÀÙ°ú ÁÙ±âÁ¶Á÷À» Ä¡»óÇÑ °á°ú ½ÅÃʼö¿Í ±æÀÌ´Â MS¹èÁö¿¡ Ä¡»óÇßÀ» ¶§¿Í ¸¶Âù°¡Áö·Î 2.4-D 0.01mg/l¿Í TDZ 0.1, 2.0(${mu}M$)ÀÇ Á¶ÇÕ󸮿¡¼­ ½ÅÃʺÐÈ­°¡ ÀϾ´Ù.
When the leaf and stem tissues of Rehmannia glutinosa were cultured on MS medium with plant growth regulators, shoots were regenerated on MS medium with TDZ(0.01 to $2.0{mu}M$), and root initiation was better on MS medium treated with NAA(0.01 to 2.0mg/$iota$). On $B_5$ medium, shoot regeneration was better on medium with TDZ than those with Quincrac, NAA, and 2.4-D. The addition of Quincrac, NAA, and 2.4-D inhibited shoot regeneration on MS medium, but promoted shoot regeneration on $B_5$ medium. Shoot regeneration and growth was better on MS medium with the combination treatments of 2.4-D 0.01mg/$iota$ and TDZ 0.01, 0.1 and $2{mu}M$ compared with other treatments. Also shoot regeneration and growth on B_5 medium showed the similar results to that on MS medium.
 
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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.10, no.1, 1997³â, pp.94-99
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199711920278611)
¾ð¾î : Çѱ¹¾î
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