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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.10, no.2, 1997³â, pp.188-193
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( The Study of Superoxide dismutase (SOD) and SOD-mimic Compounds in Panax ginseng C.A.Meyer ) |
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| °í·ÁÀλï 1³â±Ù¿¡¼ 5³â±ÙÀ» Àü±â¿µµ¿ÇÏ¿´°í SOD Ȱ¼º¿¡ ´ëÇÑ ¿°»öÀ» ¼öÇàÇÏ¿´´Ù. ±× °á°ú ÃÑ 13°³ÀÇ ±¸ºÐµÇ¾îÁö´Â ¹«»öÀÇ ¹êµå¸¦ ³ªÅ¸³»¾ú´Ù. ±×¸®°í ¹êµåÀÇ ÆÐÅÏÀº 1³â±Ù¿¡¼ 5³â±ÙÀÌ ¸ðµÎ µ¿ÀÏ ÇÏ¿´´Ù. À̵é È¿¼Ò ÁßÀÇ 9°³´Â ½Ã¾Æ³ªÀ̵å 󸮳ª °ú»êȼö¼Ò 󸮿¡ ÀÇÇØ ¹êµå°¡ Á¦°ÅµÇ¾ú°í Ŭ·Î·ÎÆ÷¸§°ú ¿¡Åº¿Ã󸮿¡ ´ëÇØ¼´Â ¾ÈÁ¤Çß´Ù. À̵é È¿¼ÒµéÀº CuZnSOD È¿¼ÒµéÀÌ´Ù. ¹Ý¸é¿¡ 4°³ÀÇ ¹êµå´Â ½Ã¾Æ³ªÀ̵å󸮳ª °ú»êȼö¼Ò 󸮿¡ ÀÇÇØ ¾ÈÁ¤Çϰí Ŭ·Î·ÎÆ÷¸§°ú ¿¡Åº¿Ã 󸮿¡ ÀÇÇØ¼´Â Á¦°ÅµÇ¾ú´Ù. ±×·¯¹Ç·Î À̵éÀº MnSOD È¿¼ÒµéÀÌ´Ù. Àλï 1³â±Ù¿¡¼ 5³â±Ù±îÁö SOD Ȱ¼ºÀÇ ºñ±³¸¦ ±¤ÈÇÐÀû ¿¡¼¼ÀÌ ¹æ¹ý¿¡ ÀÇÇØ ÃøÁ¤ÇÏ¿´´Ù. À̵é SOD Ȱ¼º ÃøÁ¤ÀÇ °á°ú¿¡¼ ³â¼ö¿¡ µû¸¥ SOD Ȱ¼ºÀÇ À¯ÀǼºÀÖ´Â Â÷ÀÌ´Â º¸¿©ÁöÁö ¾Ê¾Ò´Ù. ¸ðµç Àλï ÃßÃâ¹°ÀÇ ÃÑ SOD Ȱ¼ºÀº ´ë·« 700-800units/g of fresh weight À̾ú´Ù. ±×·¯³ª SOD È¿¼Ò¿¡ ÀÇÇØ SOD Ȱ¼ºÀº ´ë·« 200-250 units/g of fresh weight À̾ú´Ù. ±×·¯¹Ç·Î SOD Ȱ¼º À¯»ç ¹°Áú¿¡ ÀÇÇØ Ȱ¼ºÀÌ SOD È¿¼Ò¿¡ ÀÇÇÑ È°¼ºº¸´Ù ³ô´Ù. SOD Ȱ¼º À¯»ç ¹°Áú°ú SOD È¿¼Ò¿¡ ÀÇÇÑ È°¼ºÀÇ ºñ´Â ´ë·« 2:1¿¡¼ 3:1ÀÌ´Ù. |
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| Panax ginseng C.A.Meyer,1 to 5 years old were electrophored and were stained for SOD activity. The result indicated a total of 13 distinct form of the enzyme and the pattern of achromatic bands were not different according to ages. Nine of the enzyme activities were eliminated with cyanide or peroxide treatment and were resistant to treatment of chloroform plus ethanol. It suggested that they may be cupro-zinc containing SOD, whereas four were cyanide or peroxide resistant and were eliminated with cholroform plus ethanol treatment. They may be manganese containing SOD. Ginseng roos. 1 to 5 years old were analyzed for their SOD measurement of SOD activities of all extracts, the significant difference of SOD activities were not shown according to ages. All ginseng extracts had the total SOD activities of all extracts, the significant difference of SOD activities were not shown according to ages. All ginseng extracts had the total SOD activities of about 700-800 unit/g of fresh weight. Therefore, the SOD activities from SOD-mimic compounds were higher than one from SOD. The ratio between the SOD activity from SOD-mimic compounds and one from true SOD was approximately 2:1 to 3:1. |
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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.10, no.2, 1997³â, pp.188-193
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199711920278726)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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