|
|
|
한국자원식물학회 / v.11, no.2, 1998년, pp.195-201 
|
흰나리 인편으로부터 PR-Proteinase의 유도 및 특성 규명
( Induction by Salicylic Acid and Characterization of PR-Proteinases from Bulbs of Lilium formosanum Wallace ) |
| 오순자; 제주대학교 생물학과;
|
|
|
 |
|
| |
| 초 록 |
|
|
| Some morphological character were surveyed and PR-proteinases were induced and characterized from Lilium formosanum Wallace endeimc to Cheju island . Its flower characters were similar to those of white trumpet lilies(Lilium longiflorum Thunb) although its flowering period was later than that of white trumpet lilies and it hadd a wide range of variation among individuals. Six PR-proteinases(II-2, III-1, III-2, IV-1, IV-2 and V) were induced from bulbs by 2.5mM salicylic acid and almost excreted into the intercellular spaces. These PR-proteinases were strongly activated by Ca 2+, , whereas they were strongly inhibited by Cu2+ Co2+ and Fe2+ . Three PR proteinases(II-2, IV-1 and IV-2) were strongly inhibited by 1, 10 -phenanthroline, indicating that these enzymes are metallo-proteinases. Three PR-proteinases(III-1, III-2 and V) had a high sensitivity to PMSF and required $eta$-mercaptoethanol for their activities. These results indicate that these proteinases are cysteine proteinases. |
| |
| 키워드 |
| PR-proteinases;salicylic acid;metallo-proteinases;cysteine proteinases;Lilium formosanum; |
| |
|
|
|
|
한국자원식물학회지 / v.11, no.2, 1998년, pp.195-201
한국자원식물학회
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199811920280016)
언어 : 한국어 |
|
| 논문 제공 : KISTI 한국과학기술정보연구원 |
|
|
|
|
|
|
